IR@PKUHSC  > 北京大学基础医学院  > 细胞生物学系
学科主题基础医学
Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imaging
Liu, Kaiyu1; Liu, Xujie2; Peng, Zhiping2; Sun, Haojie3,4; Zhang, Mingzhi3,4; Zhang, Jianning5; Liu, Shuang5; Hao, Limin6; Lu, Guoqiu6; Zheng, Kangcheng6; Gong, Xikui6; Wu, Di6; Wang, Fan7,8; Shen, Li3,4
关键词adoptive immunotherapy avidin-CAR biotinylated EGFRvIII optical imaging
刊名ONCOTARGET
2015-09-15
DOI10.18632/oncotarget.4362
6期:27页:23735-23747
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Cell Biology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Oncology ; Cell Biology
关键词[WOS]GROWTH-FACTOR RECEPTOR ; CHIMERIC ANTIGEN RECEPTOR ; ANTITUMOR-ACTIVITY ; GLIOBLASTOMA-MULTIFORME ; CANCER ; THERAPY ; TUMORIGENICITY ; NEUROBLASTOMA ; CONTRIBUTES ; LYMPHOCYTES
英文摘要

There has been significant progress in the design of chimeric antigen receptors (CAR) for adoptive immunotherapy targeting tumor-associated antigens. However, the challenge of monitoring the therapy in real time has been continually ignored. To address this issue, we developed optical molecular imaging approaches to evaluate a recently reported novel CAR strategy for adoptive immunotherapy against glioma xenografts expressing EGFRvIII. We initially biotinylated a novel anti-EGFRvIII monoclonal antibody (biotin-4G1) to pre-target EGFRvIII(+) gliomas and then redirect activated avidin-CAR expressing T cells against the pre-targeted biotin-4G1. By optical imaging study and bio-distribution analysis, we confirmed the specificity of pre-target and target and determined the optimal time for T cells adoptive transfer in vivo. The results showed this therapeutic strategy offered efficient therapy effect to EGFRvIII(+) glioma-bearing mice and implied that optical imaging is a highly useful tool in aiding in the instruction of clinical CAR-T cells adoptive transfer in future.

语种英语
所属项目编号81272790
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000363157700051
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65777
专题北京大学基础医学院_细胞生物学系
作者单位1.Peking Univ, Stem Cell Res Ctr, Beijing 100871, Peoples R China
2.Chinese PLA Navy Gen Hosp, Dept Neurosurg, Beijing, Peoples R China
3.Beijing Cellonis Biotechnol Co Ltd, Beijing, Peoples R China
4.Peking Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Beijing 100871, Peoples R China
5.Chongqing Med Univ, Dept Med Radiol, Chongqing, Peoples R China
6.Peking Univ, Dept Cell Biol, Hlth Sci Ctr, Beijing 100871, Peoples R China
7.Peking Univ, Med Isotopes Res Ctr, Beijing 100871, Peoples R China
8.Peking Univ, Dept Radiat Med, Sch Basic Med Sci, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Liu, Kaiyu,Liu, Xujie,Peng, Zhiping,et al. Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imaging[J]. ONCOTARGET,2015,6(27):23735-23747.
APA Liu, Kaiyu.,Liu, Xujie.,Peng, Zhiping.,Sun, Haojie.,Zhang, Mingzhi.,...&Shen, Li.(2015).Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imaging.ONCOTARGET,6(27),23735-23747.
MLA Liu, Kaiyu,et al."Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imaging".ONCOTARGET 6.27(2015):23735-23747.
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