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学科主题: 药学
题名:
Comprehensive Chemical Analysis of the Rhizomes of Drynaria fortunei by Orthogonal Pre-Separation and Liquid Chromatography Mass Spectrometry
作者: Qiao, Xue1; Lin, Xiong-hao1; Liang, Yong-hong1; Dong, Jing2; Guo, De-an1; Ye, Min1
关键词: Drynaria fortune ; Polypodiaceae ; pre-separation ; proanthocyanidins ; orthogonal chromatography ; HPLC-DAD-ESI-MSn
刊名: PLANTA MEDICA
发表日期: 2014-03-01
DOI: 10.1055/s-0033-1360362
卷: 80, 期:4, 页:330-336
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]: PROANTHOCYANIDINS
英文摘要:

The chemical composition of Drynaria fortunei, a traditional Chinese herbal medicine, is very complicated. In order to separate these chemicals to obtain their structural information, an orthogonal sample enrichment procedure was established. The ethyl acetate extract of D. fortunei was pre-separated by Sephadex LH-20 x polyamide columns to yield 15 fractions. These fractions were analyzed successively using a reversed-phase Agilent Zorbax SB-C-18 column, coupled with diode array detection and electrospray ionization tandem mass spectrometry. The method reduced co-elution and enriched minor compounds on the basis of their chemical features. A total of 369 compounds were detected by LC/MSn, compared to less than 50 compounds without pre-separation. The pretreatment facilitated the analytical separation of flavonoids, proanthocyanidins, triterpenoids, phenolic acids, and lignans in D. fortunei, and allowed a comprehensive chemical profiling of these constituents. This method could be applied to other multicomponent herbal extracts.

语种: 英语
所属项目编号: 81222054 ; NCET-11-0019
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from the Chinese Ministry of Education
WOS记录号: WOS:000332395000012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65779
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Shimadzu Int Trading Shanghai Co Ltd, Beijing Off, Beijing, Peoples R China

Recommended Citation:
Qiao, Xue,Lin, Xiong-hao,Liang, Yong-hong,et al. Comprehensive Chemical Analysis of the Rhizomes of Drynaria fortunei by Orthogonal Pre-Separation and Liquid Chromatography Mass Spectrometry[J]. PLANTA MEDICA,2014,80(4):330-336.
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