IR@PKUHSC  > 北京大学口腔医学院  > 牙周科
学科主题口腔医学
The expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1 alpha,25-dihydroxyvitamin D-3
Zhang, D; Yang, YQ; Li, XT; Fu, MK
关键词human periodontal ligament cells OPG RANKL 1 alpha,25(OH)(2)vitD(3)
刊名ARCHIVES OF ORAL BIOLOGY
2004
DOI10.1016/S0003-9969(03)00201-2
49期:1页:71-76
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]OSTEOCLASTOGENESIS-INHIBITORY FACTOR ; DIFFERENTIATION FACTOR ; BONE ; FIBROBLASTS ; MECHANISM ; OPG
英文摘要

The receptor activator of nuclear factor kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are important bone metabolism molecules, which directly control osteoclastogenesis. Periodontal. ligament (PDL) cells play a vital role in maintaining the homeostasis of periodontal tissues, releasing cytokines to affect bone metabolism. The purpose of this study was to investigate the expression of OPG and RANKL in cultured human periodontal. ligament cells (hPDLCs) derived from permanent teeth and the expression change after stimulation by 1alpha,25-dihydroxyvitamin D-3 (1alpha,25(OH)(2)vitD(3)), a kind of bone resorption promoter. HPDLCs were cultured in the presence or absence of 10(-8) M 1alpha,25(OH)(2)vitD(3) in vitro. The expression of mRNA for OPG and RANKL in hPDLCs during 6 days′ culture was examined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The level of secreted OPG protein in the culture medium during 6 days′ culture was detected by enzyme-linked immunoabsorbent assay (ELISA). The result showed that OPG and RANKL were expressed by hPDLCs. OPG expression was down-regulated by 10(-8)M 1alpha,25(OH)(2)vitD(3) in a time-dependent manner, while RANKL mRNA was up-regulated. The ratio of OPG/RANKL was decreased. In conclusion, our findings suggest that hPDLCs may regulate the alveolar bone metabolism through the OPG/RANKL system. (C) 2003 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000188418200009
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65780
专题北京大学口腔医学院_牙周科
作者单位Peking Univ, Sch Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Zhang, D,Yang, YQ,Li, XT,et al. The expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1 alpha,25-dihydroxyvitamin D-3[J]. ARCHIVES OF ORAL BIOLOGY,2004,49(1):71-76.
APA Zhang, D,Yang, YQ,Li, XT,&Fu, MK.(2004).The expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1 alpha,25-dihydroxyvitamin D-3.ARCHIVES OF ORAL BIOLOGY,49(1),71-76.
MLA Zhang, D,et al."The expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1 alpha,25-dihydroxyvitamin D-3".ARCHIVES OF ORAL BIOLOGY 49.1(2004):71-76.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, D]的文章
[Yang, YQ]的文章
[Li, XT]的文章
百度学术
百度学术中相似的文章
[Zhang, D]的文章
[Yang, YQ]的文章
[Li, XT]的文章
必应学术
必应学术中相似的文章
[Zhang, D]的文章
[Yang, YQ]的文章
[Li, XT]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。