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学科主题: 口腔医学
题名:
The expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1 alpha,25-dihydroxyvitamin D-3
作者: Zhang, D; Yang, YQ; Li, XT; Fu, MK
关键词: human periodontal ligament cells ; OPG ; RANKL ; 1 alpha,25(OH)(2)vitD(3)
刊名: ARCHIVES OF ORAL BIOLOGY
发表日期: 2004
DOI: 10.1016/S0003-9969(03)00201-2
卷: 49, 期:1, 页:71-76
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: OSTEOCLASTOGENESIS-INHIBITORY FACTOR ; DIFFERENTIATION FACTOR ; BONE ; FIBROBLASTS ; MECHANISM ; OPG
英文摘要:

The receptor activator of nuclear factor kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are important bone metabolism molecules, which directly control osteoclastogenesis. Periodontal. ligament (PDL) cells play a vital role in maintaining the homeostasis of periodontal tissues, releasing cytokines to affect bone metabolism. The purpose of this study was to investigate the expression of OPG and RANKL in cultured human periodontal. ligament cells (hPDLCs) derived from permanent teeth and the expression change after stimulation by 1alpha,25-dihydroxyvitamin D-3 (1alpha,25(OH)(2)vitD(3)), a kind of bone resorption promoter. HPDLCs were cultured in the presence or absence of 10(-8) M 1alpha,25(OH)(2)vitD(3) in vitro. The expression of mRNA for OPG and RANKL in hPDLCs during 6 days′ culture was examined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The level of secreted OPG protein in the culture medium during 6 days′ culture was detected by enzyme-linked immunoabsorbent assay (ELISA). The result showed that OPG and RANKL were expressed by hPDLCs. OPG expression was down-regulated by 10(-8)M 1alpha,25(OH)(2)vitD(3) in a time-dependent manner, while RANKL mRNA was up-regulated. The ratio of OPG/RANKL was decreased. In conclusion, our findings suggest that hPDLCs may regulate the alveolar bone metabolism through the OPG/RANKL system. (C) 2003 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000188418200009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65780
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: Peking Univ, Sch Stomatol, Dept Orthodont, Beijing 100081, Peoples R China

Recommended Citation:
Zhang, D,Yang, YQ,Li, XT,et al. The expression of osteoprotegerin and the receptor activator of nuclear factor kappa B ligand in human periodontal ligament cells cultured with and without 1 alpha,25-dihydroxyvitamin D-3[J]. ARCHIVES OF ORAL BIOLOGY,2004,49(1):71-76.
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