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Influence of substrate on corneal epithelial cell viability within ocular surface models
Feng, Yun1,2; Foster, James1; Mi, Shengli1,3; Chen, Bo1; Connon, Che John1
关键词tissue engineering cornea toxicity collagen model
刊名EXPERIMENTAL EYE RESEARCH
2012-08-01
DOI10.1016/j.exer.2012.05.005
101页:97-103
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]EYE IRRITATION TESTS ; ORGAN-CULTURE MODEL ; INTERLABORATORY VALIDATION ; COSMETIC INGREDIENTS ; PLASTIC COMPRESSION ; ELECTRON MICROSCOPY ; STEM-CELLS ; COLLAGEN ; SCAFFOLD ; PRODUCTS
英文摘要

Corneal tissue engineering has improved dramatically over recent years. It is now possible to apply these technological advancements to the development of superior in vitro ocular surface models to reduce animal testing. We aim to show the effect different substrates can have on the viability of expanded corneal epithelial cells and that those which more accurately mimic the stromal surface provide the most protection against toxic assault. Compressed collagen gel as a substrate for the expansion of a human epithelial cell line was compared against two well-known substrates for modelling the ocular surface (polycarbonate membrane and conventional collagen gel). Cells were expanded over 10 days at which point cell stratification, cell number and expression of junctional proteins were assessed by electron microscopy, immunohistochemistry and RT-PCR. The effect of increasing concentrations of sodium lauryl sulphate on epithelial cell viability was quantified by MTT assay. Results showed improvement in terms of stratification, cell number and tight junction expression in human epithelial cells expanded upon either the polycarbonate membrane or compressed collagen gel when compared to a the use of a conventional collagen gel. However, cell viability was significantly higher in cells expanded upon the compressed collagen gel. We conclude that the more naturalistic composition and mechanical properties of compressed collagen gels produces a more robust corneal model. (C) 2012 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000307205300013
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65781
专题北京大学第三临床医学院_眼科
作者单位1.Univ Reading, Sch Pharm, Reading RG6 2AH, Berks, England
2.Peking Univ, Hosp 3, Ctr Eye, Beijing, Peoples R China
3.Tsinghua Univ, Grad Sch, Shenzhen 518057, Peoples R China
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GB/T 7714
Feng, Yun,Foster, James,Mi, Shengli,et al. Influence of substrate on corneal epithelial cell viability within ocular surface models[J]. EXPERIMENTAL EYE RESEARCH,2012,101:97-103.
APA Feng, Yun,Foster, James,Mi, Shengli,Chen, Bo,&Connon, Che John.(2012).Influence of substrate on corneal epithelial cell viability within ocular surface models.EXPERIMENTAL EYE RESEARCH,101,97-103.
MLA Feng, Yun,et al."Influence of substrate on corneal epithelial cell viability within ocular surface models".EXPERIMENTAL EYE RESEARCH 101(2012):97-103.
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