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学科主题: 基础医学
题名:
Kirenol, a compound from Herba Siegesbeckiae, induces apoptosis in human chronic myeloid leukemia K562 cells
作者: Lu, Yue1; Qian, Ruiqin1; Xiao, Juan2; Xu, Dong2; Fu, Hongzheng3; Chen, Yingyu2
刊名: PHARMAZIE
发表日期: 2014-02-01
DOI: 10.1691/ph.2014.3560
卷: 69, 期:2, 页:148-153
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy ; Chemistry
关键词[WOS]: CYCLIN-DEPENDENT KINASES ; BCL-2 FAMILY PROTEINS ; CYTOCHROME-C ; P53 ; P21 ; MITOCHONDRIA ; INHIBITION ; MECHANISMS ; RELEASE ; CANCER
英文摘要:

Kirenol is a biologically active substance isolated from Herba Siegesbeckiae. In the experiments, we explored a novel antitumor activity of kirenol. The data demonstrated that kirenol had strong cytotoxic effects to human chronic myeloid leukemia K562 cells, the 50% inhibitory concentration (IC50) for kirenol was 53.05 mu g/ml,18.19 mu g/ml and 15.08 mu g/ml for 24, 48 and 72 h, determined using the MU assay. Further studies showed that kirenol treatment caused externalization of phosphatidylserine, accumulation of ROS (reactive oxygen species), alteration of mitochondrial membrane potential, release of cytochrome c, reduction in the level of the Bcl-2 protein and upregulation of Bax and tBid, kirenol induced cell apoptosis in a caspase-independent manner. Further studies indicated that kirenol treatment triggered the arrest of cell cycle S period which might resulted from the up-regulation of phosphorylation of p53 (Ser 6 and Ser 37) and expression of p21 protein. Our results indicated that kirenol possesses antitumor action on human chronic myeloid leukemia K562 cells in vitro. Kirenol may have therapeutic potential for the treatment of cancer that deserves further investigation.

语种: 英语
所属项目编号: 2011CB910103 ; 2012ZX09101 ; 7072043
项目资助者: National Key Project for Basic Research of China (973) ; National Key New Drug Creation Program of China ; Natural Science Foundation of Beijing, China
WOS记录号: WOS:000334261000013
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65789
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Dept Integrated Chinese Tradit Western Med, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Dept Immunol, Ctr Human Dis Genom, Beijing 100191, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Lu, Yue,Qian, Ruiqin,Xiao, Juan,et al. Kirenol, a compound from Herba Siegesbeckiae, induces apoptosis in human chronic myeloid leukemia K562 cells[J]. PHARMAZIE,2014,69(2):148-153.
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