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学科主题: 临床医学
题名:
Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs
作者: Sun, Yi1; Yu, Shuang2; Sun, Peng3; Wu, Haiyan3; Zhu, Weidong4; Liu, Wei1; Zhang, Jue2,3; Fang, Jing2,3; Li, Ruoyu1
刊名: PLOS ONE
发表日期: 2012-07-10
DOI: 10.1371/journal.pone.0040629
卷: 7, 期:7
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: PRESSURE AIR PLASMA ; INVASIVE CANDIDIASIS ; ALBICANS BIOFILMS ; HYDROGEN-PEROXIDE ; SINGLET OXYGEN ; RESISTANCE ; COAGULATION ; EPIDEMIOLOGY ; MICROJET ; BACTERIA
英文摘要:

We investigated the antifungal effect of non-thermal plasma, as well as its combination with common antifungal drugs, against Candida biofilms. A direct current atmospheric pressure He/O-2 (2%) plasma microjet (PMJ) was used to treat Candida biofilms in a 96-well plate. Inactivation efficacies of the biofilms were evaluated by XTT assay and counting colony forming units (CFUs). Morphological properties of the biofilms were evaluated by Scanning Electron Microscope (SEM). The sessile minimal inhibitory concentrations (SMICs) of fluconazole, amphotericin B, and caspofungin for the biofilms were also tested. Electron Spin Resonance (ESR) spectroscopy was used to detect the reactive oxygen species (ROS) generated directly and indirectly by PMJ. The Candida biofilms were completely inactivated after 1 min PMJ treatment, where severely deformed fungal elements were observed in SEM images. The SMICs of the tested antifungal drugs for the plasma-treated biofilms were decreased by 2-6 folds of dilution, compared to those of the untreated controls. ROS such as hydroxyl radical ((OH)-O-center dot), superoxide anion radical (O-center dot(2)) and singlet molecular oxygen (O-1(2)) were detected by ESR. We hence conclude that He/O-2 (2%) plasma alone, as well as in combination with common antifungal drugs, is able to inactivate Candida biofilms rapidly. The generation of ROS is believed to be one of the underlying mechanisms for the fungicidal activity

语种: 英语
所属项目编号: 30970131 ; NCET-10-0198 ; 7102149
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Natural Science Foundation
WOS记录号: WOS:000306355500064
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65793
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Peking Univ, Dept Dermatol & Venereolgy, Hosp 1, Res Ctr Med Mycol, Beijing 100871, Peoples R China
2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
3.Peking Univ, Coll Engn, Beijing 100871, Peoples R China
4.St Peters Coll, Dept Appl Sci & Technol, Jersey City, NJ USA

Recommended Citation:
Sun, Yi,Yu, Shuang,Sun, Peng,et al. Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs[J]. PLOS ONE,2012,7(7).
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