IR@PKUHSC  > 北京大学第三临床医学院  > 泌尿外科
学科主题临床医学
Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs
Sun, Yi1; Yu, Shuang2; Sun, Peng3; Wu, Haiyan3; Zhu, Weidong4; Liu, Wei1; Zhang, Jue2,3; Fang, Jing2,3; Li, Ruoyu1
刊名PLOS ONE
2012-07-10
DOI10.1371/journal.pone.0040629
7期:7
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]PRESSURE AIR PLASMA ; INVASIVE CANDIDIASIS ; ALBICANS BIOFILMS ; HYDROGEN-PEROXIDE ; SINGLET OXYGEN ; RESISTANCE ; COAGULATION ; EPIDEMIOLOGY ; MICROJET ; BACTERIA
英文摘要

We investigated the antifungal effect of non-thermal plasma, as well as its combination with common antifungal drugs, against Candida biofilms. A direct current atmospheric pressure He/O-2 (2%) plasma microjet (PMJ) was used to treat Candida biofilms in a 96-well plate. Inactivation efficacies of the biofilms were evaluated by XTT assay and counting colony forming units (CFUs). Morphological properties of the biofilms were evaluated by Scanning Electron Microscope (SEM). The sessile minimal inhibitory concentrations (SMICs) of fluconazole, amphotericin B, and caspofungin for the biofilms were also tested. Electron Spin Resonance (ESR) spectroscopy was used to detect the reactive oxygen species (ROS) generated directly and indirectly by PMJ. The Candida biofilms were completely inactivated after 1 min PMJ treatment, where severely deformed fungal elements were observed in SEM images. The SMICs of the tested antifungal drugs for the plasma-treated biofilms were decreased by 2-6 folds of dilution, compared to those of the untreated controls. ROS such as hydroxyl radical ((OH)-O-center dot), superoxide anion radical (O-center dot(2)) and singlet molecular oxygen (O-1(2)) were detected by ESR. We hence conclude that He/O-2 (2%) plasma alone, as well as in combination with common antifungal drugs, is able to inactivate Candida biofilms rapidly. The generation of ROS is believed to be one of the underlying mechanisms for the fungicidal activity

语种英语
WOS记录号WOS:000306355500064
项目编号30970131 ; NCET-10-0198 ; 7102149
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Beijing Natural Science Foundation
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65793
专题北京大学第三临床医学院_泌尿外科
北京大学第一临床医学院_皮肤性病科
作者单位1.Peking Univ, Dept Dermatol & Venereolgy, Hosp 1, Res Ctr Med Mycol, Beijing 100871, Peoples R China
2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
3.Peking Univ, Coll Engn, Beijing 100871, Peoples R China
4.St Peters Coll, Dept Appl Sci & Technol, Jersey City, NJ USA
推荐引用方式
GB/T 7714
Sun, Yi,Yu, Shuang,Sun, Peng,et al. Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs[J]. PLOS ONE,2012,7(7).
APA Sun, Yi.,Yu, Shuang.,Sun, Peng.,Wu, Haiyan.,Zhu, Weidong.,...&Li, Ruoyu.(2012).Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs.PLOS ONE,7(7).
MLA Sun, Yi,et al."Inactivation of Candida Biofilms by Non-Thermal Plasma and Its Enhancement for Fungistatic Effect of Antifungal Drugs".PLOS ONE 7.7(2012).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sun, Yi]的文章
[Yu, Shuang]的文章
[Sun, Peng]的文章
百度学术
百度学术中相似的文章
[Sun, Yi]的文章
[Yu, Shuang]的文章
[Sun, Peng]的文章
必应学术
必应学术中相似的文章
[Sun, Yi]的文章
[Yu, Shuang]的文章
[Sun, Peng]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。