IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心
学科主题临床医学
Promotion of Human Early Embryonic Development and Blastocyst Outgrowth In Vitro Using Autocrine/Paracrine Growth Factors
Kawamura, Kazuhiro1,2; Chen, Yuan3; Shu, Yimin1; Cheng, Yuan1; Qiao, Jie3; Behr, Barry1; Pera, Renee A. Reijo1; Hsueh, Aaron J. W.1
刊名PLOS ONE
2012-11-12
DOI10.1371/journal.pone.0049328
7期:11
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Uehara Memorial Foundation ; Naito Foundation ; Mochida Memorial Foundation for Medical and Pharmaceutical Research ; Major State Basic Research Development Program of China ; National Natural Sciences Foundation of China ; California Institute of Regenerative Medicine (CIRM) ; Uehara Memorial Foundation ; Naito Foundation ; Mochida Memorial Foundation for Medical and Pharmaceutical Research ; Major State Basic Research Development Program of China ; National Natural Sciences Foundation of China ; California Institute of Regenerative Medicine (CIRM)
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]RANDOMIZED CLINICAL-TRIAL ; PREIMPLANTATION EMBRYO ; NEUROTROPHIC FACTOR ; MOUSE EMBRYOS ; HUMAN OOCYTES ; IMPLANTATION ; EXPRESSION ; ALPHA ; IVF ; FERTILIZATION
英文摘要

Studies using animal models demonstrated the importance of autocrine/paracrine factors secreted by preimplantation embryos and reproductive tracts for embryonic development and implantation. Although in vitro fertilization-embryo transfer (IVF-ET) is an established procedure, there is no evidence that present culture conditions are optimal for human early embryonic development. In this study, key polypeptide ligands known to be important for early embryonic development in animal models were tested for their ability to improve human early embryo development and blastocyst outgrowth in vitro. We confirmed the expression of key ligand/receptor pairs in cleavage embryos derived from discarded human tri-pronuclear zygotes and in human endometrium. Combined treatment with key embryonic growth factors (brain-derived neurotrophic factor, colony-stimulating factor, epidermal growth factor, granulocyte macrophage colony-stimulating factor, insulin-like growth factor-1, glial cell-line derived neurotrophic factor, and artemin) in serum-free media promoted >2.5-fold the development of tri-pronuclear zygotes to blastocysts. For normally fertilized embryos, day 3 surplus embryos cultured individually with the key growth factors showed >3-fold increases in the development of 6-8 cell stage embryos to blastocysts and >7-fold increase in the proportion of high quality blastocysts based on Gardner′s criteria. Growth factor treatment also led to a 2-fold promotion of blastocyst outgrowth in vitro when day 7 surplus hatching blastocysts were used. When failed-to-be-fertilized oocytes were used to perform somatic cell nuclear transfer (SCNT) using fibroblasts as donor karyoplasts, inclusion of growth factors increased the progression of reconstructed SCNT embryos to >4-cell stage embryos. Growth factor supplementation of serum-free cultures could promote optimal early embryonic development and implantation in IVF-ET and SCNT procedures. This approach is valuable for infertility treatment and future derivation of patient-specific embryonic stem cells.

语种英语
所属项目编号2011CB944504 ; 81000234 ; RC1-00137-1 ; RS1-00404 ; RB2-01553 ; 24390376 ; 23013004 ; 24659722
资助者Uehara Memorial Foundation ; Naito Foundation ; Mochida Memorial Foundation for Medical and Pharmaceutical Research ; Major State Basic Research Development Program of China ; National Natural Sciences Foundation of China ; California Institute of Regenerative Medicine (CIRM) ; Uehara Memorial Foundation ; Naito Foundation ; Mochida Memorial Foundation for Medical and Pharmaceutical Research ; Major State Basic Research Development Program of China ; National Natural Sciences Foundation of China ; California Institute of Regenerative Medicine (CIRM)
WOS记录号WOS:000311234000064
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65794
Collection北京大学第三临床医学院_生殖医学中心
作者单位1.Stanford Univ, Sch Med, Dept Obstet & Gynecol, Program Reprod & Stem Cell Biol, Stanford, CA 94305 USA
2.Akita Univ, Grad Sch Med, Dept Obstet & Gynecol, Akita 010, Japan
3.Peking Univ, Hosp 3, Ctr Reprod Med, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Kawamura, Kazuhiro,Chen, Yuan,Shu, Yimin,et al. Promotion of Human Early Embryonic Development and Blastocyst Outgrowth In Vitro Using Autocrine/Paracrine Growth Factors[J]. PLOS ONE,2012,7(11).
APA Kawamura, Kazuhiro.,Chen, Yuan.,Shu, Yimin.,Cheng, Yuan.,Qiao, Jie.,...&Hsueh, Aaron J. W..(2012).Promotion of Human Early Embryonic Development and Blastocyst Outgrowth In Vitro Using Autocrine/Paracrine Growth Factors.PLOS ONE,7(11).
MLA Kawamura, Kazuhiro,et al."Promotion of Human Early Embryonic Development and Blastocyst Outgrowth In Vitro Using Autocrine/Paracrine Growth Factors".PLOS ONE 7.11(2012).
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