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Levo-tetrahydropalmatine attenuates mouse blood-brain barrier injury induced by focal cerebral ischemia and reperfusion: Involvement of Src kinase
Mao, Xiao-Wei1,2; Pan, Chun-Shui2,3,4; Huang, Ping2,3,4; Liu, Yu-Ying2,3,4; Wang, Chuan-She1,2,3,4; Yan, Li2,3,4; Hu, Bai-He2,3,4; Chang, Xin2,3,4; He, Ke2,3,4; Mu, Huan-Na2,3,4; Li, Quan2,3,4; Sun, Kai2,3,4; Fan, Jing-Yu2,3,4; Han, Jing-Yan1,2,3,4
刊名SCIENTIFIC REPORTS
2015-06-10
DOI10.1038/srep11155
5期:0
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]Matrix Metalloproteinases ; Tyrosine Phosphorylation ; Stroke ; Disruption ; Inhibition ; Activation ; Addiction ; Mechanism ; Membrane
英文摘要

The restoration of blood flow following thrombolytic therapy causes ischemia and reperfusion (I/R) injury leading to blood-brain barrier (BBB) disruption and subsequent brain edema in patients of ischemic stroke. Levo-tetrahydropalmatine (l-THP) occurs in Corydalis genus and some other plants. However, whether l-THP exerts protective role on BBB disrpution following cerebral I/R remains unclear. Male C57BL/6N mice (23 to 28 g) were subjected to 90 min middle cerebral artery occlusion, followed by reperfusion for 24 h. l-THP (10, 20, 40 mg/kg) was administrated by gavage 60 min before ischemia. We found I/R evoked Evans blue extravasation, albumin leakage, brain water content increase, cerebral blood flow decrease, cerebral infarction and neurological deficits, all of which were attenuated by l-THP treatment. Meanwhile, l-THP inhibited tight junction (TJ) proteins down-expression, Src kinase phosphorylation, matrix metalloproteinases-2/9 (MMP-2/9) and caveolin-1 activation. In addition, surface plasmon resonance revealed binding of l-THP to Src kinase with high affinity. Then we found Src kinase inhibitor PP2 could attenuate Evans blue dye extravasation and inhibit the caveolin-1, MMP-9 activation, occludin down-expression after I/R, respectively. In conclusion, l-THP attenuated BBB injury and brain edema, which were correlated with inhibiting the Src kinase phosphorylation.

语种英语
WOS记录号WOS:000356086100001
通讯作者邮箱hanjingyan@bjmu.edu.cn
项目编号2013ZX09402202
资助机构Production of New Medicine Program of Ministry of Science and Technology of China
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65802
专题北京大学基础医学院
北京大学医学部管理机构_医学部
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100871, Peoples R China
2.State Adm Tradit Chinese Med China, Key Lab Microcirculat, Beijing, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100871, Peoples R China
4.State Adm Tradit Chinese Med China, Key Lab Stasis & Phlegm, Beijing, Peoples R China
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Mao, Xiao-Wei,Pan, Chun-Shui,Huang, Ping,et al. Levo-tetrahydropalmatine attenuates mouse blood-brain barrier injury induced by focal cerebral ischemia and reperfusion: Involvement of Src kinase[J]. SCIENTIFIC REPORTS,2015,5(0).
APA Mao, Xiao-Wei.,Pan, Chun-Shui.,Huang, Ping.,Liu, Yu-Ying.,Wang, Chuan-She.,...&Han, Jing-Yan.(2015).Levo-tetrahydropalmatine attenuates mouse blood-brain barrier injury induced by focal cerebral ischemia and reperfusion: Involvement of Src kinase.SCIENTIFIC REPORTS,5(0).
MLA Mao, Xiao-Wei,et al."Levo-tetrahydropalmatine attenuates mouse blood-brain barrier injury induced by focal cerebral ischemia and reperfusion: Involvement of Src kinase".SCIENTIFIC REPORTS 5.0(2015).
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