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学科主题: 公共卫生
题名:
Changes in the brain mitochondrial proteome of male Sprague-Dawley rats treated with manganese chloride
作者: Zhang, SR; Fu, JL; Zhou, ZC
关键词: manganese chloride ; mitochondria ; 2D-PAGE ; MALDI-TOF
刊名: TOXICOLOGY AND APPLIED PHARMACOLOGY
发表日期: 2005
DOI: 10.1016/j.taap.2004.06.001
卷: 202, 期:1, 页:13-17
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: MUTAGENESIS ; INDUCTION ; TOXICITY ; EXPOSURE ; CALCIUM ; YEAST ; CELLS
英文摘要:

To probe the mitochondrial involvement in Mn intoxicity, aliquots of brain mitochondria samples from control and treated (30 mg/kg manganese chloride, ip) male Sprague-Dawley rats were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and searched for protein abundance changes induced by Mn exposure. The electrophoretic separation resolved over 300 distinct spots as visualized by colloidal Coomassie blue (CCB), of which three spots were induced and three spots were inhibited after Mn exposure in all the five brain mitochondria preparations. Analysis by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) indicated that these spots are calcium-transporting ATPase type 2C (ATP-dependent Ca2+ pump PMRI); 60-kDa heat shock protein; Mitochondrial transmembrane GTPase FZOIB; ATP-binding cassette, sub-family b; Long-chain-fatty-acid-CoA ligase; ATP Synthase Beta Chain; and Succinate dehydrogenase flavoprotein subunit. The changes of the mitochondrial ATP synthase beta-subunit and Succinate dehydrogenase flavoprotein subunit indicate an effected level of mitochondrial ATP content and/or ATP-producing capacity. This result provides suggestion that respiratory chain complexes were implicated in the mitochondrial dysfunction induced by Mn intoxicity. And the changes of 60-kDa heat shock protein and ATP-dependent Ca2+ pump PMRI expression indicate that the Ca homeostasis and stress effect were involved in Mn intoxicity. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000226379300002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65805
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Dept Toxicol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, SR,Fu, JL,Zhou, ZC. Changes in the brain mitochondrial proteome of male Sprague-Dawley rats treated with manganese chloride[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2005,202(1):13-17.
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