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Changes in the brain mitochondrial proteome of male Sprague-Dawley rats treated with manganese chloride
Zhang, SR; Fu, JL; Zhou, ZC
关键词manganese chloride mitochondria 2D-PAGE MALDI-TOF
刊名TOXICOLOGY AND APPLIED PHARMACOLOGY
2005
DOI10.1016/j.taap.2004.06.001
202期:1页:13-17
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Toxicology
关键词[WOS]MUTAGENESIS ; INDUCTION ; TOXICITY ; EXPOSURE ; CALCIUM ; YEAST ; CELLS
英文摘要

To probe the mitochondrial involvement in Mn intoxicity, aliquots of brain mitochondria samples from control and treated (30 mg/kg manganese chloride, ip) male Sprague-Dawley rats were separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and searched for protein abundance changes induced by Mn exposure. The electrophoretic separation resolved over 300 distinct spots as visualized by colloidal Coomassie blue (CCB), of which three spots were induced and three spots were inhibited after Mn exposure in all the five brain mitochondria preparations. Analysis by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) indicated that these spots are calcium-transporting ATPase type 2C (ATP-dependent Ca2+ pump PMRI); 60-kDa heat shock protein; Mitochondrial transmembrane GTPase FZOIB; ATP-binding cassette, sub-family b; Long-chain-fatty-acid-CoA ligase; ATP Synthase Beta Chain; and Succinate dehydrogenase flavoprotein subunit. The changes of the mitochondrial ATP synthase beta-subunit and Succinate dehydrogenase flavoprotein subunit indicate an effected level of mitochondrial ATP content and/or ATP-producing capacity. This result provides suggestion that respiratory chain complexes were implicated in the mitochondrial dysfunction induced by Mn intoxicity. And the changes of 60-kDa heat shock protein and ATP-dependent Ca2+ pump PMRI expression indicate that the Ca homeostasis and stress effect were involved in Mn intoxicity. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000226379300002
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65805
专题北京大学公共卫生学院_毒理学系
作者单位Peking Univ, Hlth Sci Ctr, Sch Publ Hlth, Dept Toxicol, Beijing 100083, Peoples R China
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Zhang, SR,Fu, JL,Zhou, ZC. Changes in the brain mitochondrial proteome of male Sprague-Dawley rats treated with manganese chloride[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2005,202(1):13-17.
APA Zhang, SR,Fu, JL,&Zhou, ZC.(2005).Changes in the brain mitochondrial proteome of male Sprague-Dawley rats treated with manganese chloride.TOXICOLOGY AND APPLIED PHARMACOLOGY,202(1),13-17.
MLA Zhang, SR,et al."Changes in the brain mitochondrial proteome of male Sprague-Dawley rats treated with manganese chloride".TOXICOLOGY AND APPLIED PHARMACOLOGY 202.1(2005):13-17.
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