北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学深圳医院  > 骨关节外科  > 期刊论文
学科主题: 临床医学
题名:
Phytomolecule icaritin incorporated PLGA/TCP scaffold for steroid-associated osteonecrosis: Proof-of-concept for prevention of hip joint collapse in bipedal emus and mechanistic study in quadrupedal rabbits
作者: Qin, Ling1,2; Yao, Dong1; Zheng, Lizhen1; Liu, Wai-Ching1; Liu, Zhong1; Lei, Ming3; Huang, Le1; Xie, Xinhui1; Wang, Xinluan1,2; Chen, Yang4; Yao, Xinsheng5; Peng, Jiang1,6; Gong, He7; Griffith, James F.8; Huang, Yanping9; Zheng, Yongping9; Feng, Jian Q.10; Liu, Ying10; Chen, Shihui1; Xiao, Deming3; Wang, Daping4; Xiong, Jiangyi4; Pei, Duanqing11; Zhang, Peng2; Pan, Xiaohua12; Wang, Xiaohong13; Lee, Kwong-Man14; Cheng, Chun-Yiu1
关键词: Steroid-associated osteonecrosis ; Bipedal emus ; Hip collapse ; Bioactive composite porous scaffold ; Phytomolecule icaritin
刊名: BIOMATERIALS
发表日期: 2015-08-01
DOI: 10.1016/j.biomaterials.2015.04.038
卷: 59, 页:125-143
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: FEMORAL-HEAD OSTEONECROSIS ; MESENCHYMAL STEM-CELLS ; FINITE-ELEMENT-ANALYSIS ; ARTICULAR-CARTILAGE ; ELASTIC PROPERTIES ; PPAR-GAMMA ; BONE REGENERATION ; ADIPOGENESIS ; EXPRESSION ; NECROSIS
英文摘要:

Steroid-associated osteonecrosis (SAON) may lead to joint collapse and subsequent joint replacement. Poly lactic-co-glycolic acid/tricalcium phosphate (P/T) scaffold providing sustained release of icaritin (a metabolite of Epimedium-derived flavonoids) was investigated as a bone defect filler after surgical core-decompression (CD) to prevent femoral head collapse in a bipedal SAON animal model using emu (a large flightless bird). The underlying mechanism on SAON was evaluated using a well-established quadrupedal rabbit model. Fifteen emus were established with SAON, and CD was performed along the femoral neck for the efficacy study. In this CD bone defect, a PIT scaffold with icaritin (P/T/I group) or without icaritin (P/T group) was implanted while no scaffold implantation was used as a control. For the mechanistic study in rabbits, the effects of icaritin and composite scaffolds on bone mesenchymal stem cells (BMSCs) recruitment, osteogenesis, and anti-adipogenesis were evaluated. Our efficacy study showed that P/T/I group had the significantly lowest incidence of femoral head collapse, better preserved cartilage and mechanical properties supported by more new bone formation within the bone tunnel. For the mechanistic study, our in vitro tests suggested that icaritin enhanced the expression of osteogenesis related genes COL1 alpha, osteocalcin, RUNX2, and BMP-2 while inhibited adipogenesis related genes C/EBP-beta, PPAR-gamma, and aP2 of rabbit BMSCs. Both P/T and P/T/I scaffolds were demonstrated to recruit BMSCs both in vitro in vitro. In conclusion, both efficacy and mechanistic studies show the potential of a bioactive composite porous P/T scaffold incorporating icaritin to enhance bone defect repair after surgical CD and prevent femoral head collapse in a bipedal SAON emu model. (C) 2015 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: GHP/001/08 ; ITS/451/09FP ; CUHK-4737/10 ; 51361130034 ; 604517
项目资助者: Hong Kong Innovation and Technology Commission (ITF) ; Hong Kong Research Grants Council (GRF) ; NSFC-DG-RTD Joint Scheme ; European Union&prime ; s 7th Framework Programme (NMP-2013-EU-China)
WOS记录号: WOS:000356122700012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65820
Appears in Collections:北京大学深圳医院_骨关节外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China
2.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Translat Med R&D Ctr, Shenzhen, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Orthopaed, Shenzhen, Peoples R China
4.Second Peoples Hosp Shenzhen, Dept Orthopaed, Shenzhen, Peoples R China
5.Jinan Univ, Coll Pharm, Inst Tradit Chinese Med & Nat Prod, Guangzhou, Guangdong, Peoples R China
6.Chinese Peoples Liberat Army Gen Hosp, Orthopaed Res Inst, Beijing, Peoples R China
7.Beihang Univ, Sch Biol Sci & Med Engn, Beijing 100191, Peoples R China
8.Chinese Univ Hong Kong, Dept Imaging & Intervent Radiol, Hong Kong, Hong Kong, Peoples R China
9.Hong Kong Polytech Univ, Interdisciplinary Div Biomed Engn, Hong Kong, Hong Kong, Peoples R China
10.Texas A&M Univ, Baylor Coll Dent, Dallas, TX USA
11.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China
12.Chinese Univ Hong Kong, Lee Hysan Clin Res Labs, Hong Kong, Hong Kong, Peoples R China
13.First Peoples Hosp, Dept Orthopaed, Shenzhen, Peoples R China
14.Tsinghua Univ, Dept Mech Engn, Beijing 100084, Peoples R China

Recommended Citation:
Qin, Ling,Yao, Dong,Zheng, Lizhen,et al. Phytomolecule icaritin incorporated PLGA/TCP scaffold for steroid-associated osteonecrosis: Proof-of-concept for prevention of hip joint collapse in bipedal emus and mechanistic study in quadrupedal rabbits[J]. BIOMATERIALS,2015,59:125-143.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qin, Ling]'s Articles
[Yao, Dong]'s Articles
[Zheng, Lizhen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qin, Ling]‘s Articles
[Yao, Dong]‘s Articles
[Zheng, Lizhen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace