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Fluorescence Detection of Single-Nucleotide Polymorphism with Single-Strand Triplex-Forming DNA Probes
Li, Xinpeng; Wang, Yuan; Guo, Jiajie; Tang, Xinjing
关键词fluorescent probes oligonucleotides pyrene single-nucleotide polymorphism triplex-forming oligonucleotides
刊名CHEMBIOCHEM
2011-12-16
DOI10.1002/cbic.201100534
12期:18页:2863-2870
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Medicinal
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]LABELED MOLECULAR BEACON ; NUCLEIC-ACIDS ; EXCIMER ; HYBRIDIZATION ; PYRENE ; SEQUENCE ; APTAMER ; STEM ; RNA ; OLIGONUCLEOTIDES
英文摘要

Triple-helix-forming oligonucleotides (TFOs) are widespread in the genome and have been found in regulatory regions, especially in promoter zones and recombination hotspots of DNA. To specifically detect these polypurine sequences, we designed and synthesized two dual pyrene-labeled single-strand oligonucleotide probes (TFO-FPs) consisting of recognition, linker, and detection sequences. The hybridization processes of TFO-FPs with target polypurine oligonucleotides involve both WatsonCrick and Hoogsteen base-pairings. Through double sensing of oligonucleotide sequences, single mutations of target oligonucleotides are detected by monitoring changes in pyrene fluorescence. The high specificities of the probes are maintained over a wide temperature range without sacrifice of hybridization kinetics.

语种英语
WOS记录号WOS:000297951300024
项目编号21072015 ; NCET-10-0203
资助机构National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; Peking University State Key Laboratory
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65840
专题北京大学药学院
北京大学医学部管理机构_医学部
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Xinpeng,Wang, Yuan,Guo, Jiajie,et al. Fluorescence Detection of Single-Nucleotide Polymorphism with Single-Strand Triplex-Forming DNA Probes[J]. CHEMBIOCHEM,2011,12(18):2863-2870.
APA Li, Xinpeng,Wang, Yuan,Guo, Jiajie,&Tang, Xinjing.(2011).Fluorescence Detection of Single-Nucleotide Polymorphism with Single-Strand Triplex-Forming DNA Probes.CHEMBIOCHEM,12(18),2863-2870.
MLA Li, Xinpeng,et al."Fluorescence Detection of Single-Nucleotide Polymorphism with Single-Strand Triplex-Forming DNA Probes".CHEMBIOCHEM 12.18(2011):2863-2870.
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