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学科主题临床医学
Simultaneously detection of genomic and expression alterations in prostate cancer using cDNA microarray
Jiang, Mei1; Li, Ming2,3; Fu, Xuping1; Huang, Yan1; Qian, Hui1; Sun, Ruping1; Mao, Yumin1; Xie, Yi1; Li, Yao1
关键词prostate cancer cDNA microarray CGH expression profiling data integration
刊名PROSTATE
2008-10-01
DOI10.1002/pros.20756
68期:14页:1496-1509
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Urology & Nephrology
研究领域[WOS]Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]COPY NUMBER ALTERATIONS ; HIGH-RESOLUTION ANALYSIS ; GENE-EXPRESSION ; HEPATOCELLULAR-CARCINOMA ; INVARIANT CHAIN ; HYBRIDIZATION ; ABERRATIONS ; ARRAY ; CGH ; PROGRESSION
英文摘要

BACKGROUND. Prostate cancer is a common disease among men but the knowledge of the Prostate carcinogenesis is still limited. METHODS. cDNA microarray-based comparative genomic hybridization (CGH) and expression profiling were performed to screen the genomic and the expression changes in prostate cancer respectively. The two data were integrated to study the influence of genomic aberrations on gene expression and seek for the genes with their expression affected by the genomic aberrations. Real-time PCR was performed to evaluate the array data. RESULTS. Array-based CGH detected gains at 2q, 3p/q, 5q, 6q, 8q, 9p, 10p/q, 11q, 12p, 14q, and 19p/q and losses at 1p, 2p, 4q, 6p/q, 7p, 11p/q, 12q, 17p/q, 19p/q, and Xp/q in more than 20% prostate tumors and narrowed these aberrations. For example, the gain of 8q was mapped to five minimal regions. Novel aberrations were also identified, such as loss at Xq21.33-q22.2. Expression profiling discovered the significant biological processes involved in the prostate carcinogenesis, such as exogenous antigen presentation via MHC class II and protein ubiquitination. Integration analysis revealed a weak positive correlation between genomic copy number and gene expression level. Fifty-three genes showed their expression directly affected by the genomic aberrations possibly, including more than one member of Ras superfamily and major histocompatibility complex (MHC). These genes are involved in multiple biological processes. CONCLUSIONS. Integration of the CGH and expression data provided more information than separate analysis. Although the direct influence of genomic aberrations on gene expression seems weak, the influence can be extended by indirect regulation through a few directly affected genes. Because the influence can be persistent, the genes directly affected by the genomic aberrations may play key roles in the prostate carcinogenesis and are worth further analysis.

语种英语
WOS记录号WOS:000259654500003
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65857
专题北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Dept Urol, Hosp 1, Beijing 100871, Peoples R China
2.Fudan Univ, State Key Lab Genet Engn, Inst Genet, Sch Life Sci, Shanghai 200433, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100871, Peoples R China
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GB/T 7714
Jiang, Mei,Li, Ming,Fu, Xuping,et al. Simultaneously detection of genomic and expression alterations in prostate cancer using cDNA microarray[J]. PROSTATE,2008,68(14):1496-1509.
APA Jiang, Mei.,Li, Ming.,Fu, Xuping.,Huang, Yan.,Qian, Hui.,...&Li, Yao.(2008).Simultaneously detection of genomic and expression alterations in prostate cancer using cDNA microarray.PROSTATE,68(14),1496-1509.
MLA Jiang, Mei,et al."Simultaneously detection of genomic and expression alterations in prostate cancer using cDNA microarray".PROSTATE 68.14(2008):1496-1509.
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