IR@PKUHSC  > 北京大学第一临床医学院  > 核医学科
学科主题临床医学
Improved synthesis and biological evaluation of Tc-99m radiolabeled AMO for miRNA imaging in tumor xenografts
Kang, Lei1; Fan, Zhongyi2; Sun, Hongwei1; Feng, Yingying3; Ma, Chao1; Yan, Ping1; Zhang, Chunli1; Ma, Huan1; Hao, Pan1; Chen, Xueqi1; Zheng, Zhibing4; Xu, Xiaojie5; Wang, Rongfu1
关键词microRNA molecular imaging AMO Tc-99m radiolabel SPECT
刊名JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
2015-11-01
DOI10.1002/jlcr.3351
58期:13-14页:461-468
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Medicinal ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Chemistry
关键词[WOS]ANTISENSE OLIGONUCLEOTIDES ; IN-VIVO ; MICRORNAS ; TARGETS ; ANTAGOMIRS ; DELIVERY ; RNA
英文摘要

MicroRNAs (miRNAs) have been considered as important biomarkers for malignant tumors. In this study, we introduced an improved Tc-99m labeling method for noninvasive visualization of overexpressed miRNAs in tumor-bearing mice. Anti-miRNA-21 oligonucleotide (AMO) with partial 2-O-methyl and phosphorothioate modification was designed and chemically synthesized. After conjugated with NHS-MAG3, AMO was labeled with Tc-99m. Optimization was made to shorten reaction time and to improve labeling efficiency. Labeling efficiency was 97%, and specific activity was 2.78MBq/ng. During 12h, Tc-99m-AMO showed no significant degradation by gel electrophoresis. Its radiochemical purity was stable, between 95.8% and 99.1%. Further, Tc-99m-AMO decreased the level of miR-21 and increased the expression of PTEN protein at cellular level, shown by qRT-PCR and Western blot. Fluorescent protein labeled AMO displayed specific distribution and good stability in tumor cells. After the administration in tumor-bearing mice, Tc-99m-AMO showed more radioactive uptake in the miR-21 over-expressed tumors than scramble control. Biodistribution results further proved the significant difference of tumor uptake between Tc-99m-AMO and Tc-99m-control. Therefore, this study presents an improved method with shorten time to prepare a Tc-99m radiolabeled AMO. In addition, it supports the role of Tc-99m-AMO for noninvasive visualization of miR-21 in malignant tumors.

语种英语
WOS记录号WOS:000366056300001
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65862
专题北京大学第一临床医学院_核医学科
作者单位1.Peking Univ, Hosp 1, Dept Nucl Med, Beijing 100034, Peoples R China
2.Peoples Liberat Army Gen Hosp, Dept Oncol, Beijing 100853, Peoples R China
3.Second Artillery Gen Hosp, Dept Colorectal Surg, Beijing 100088, Peoples R China
4.Beijing Inst Pharmacol & Toxicol, Lab Comp Aided Drug Design & Discovery, Beijing 100850, Peoples R China
5.Beijing Inst Biotechnol, Dept Med Mol Biol, Beijing 100850, Peoples R China
推荐引用方式
GB/T 7714
Kang, Lei,Fan, Zhongyi,Sun, Hongwei,et al. Improved synthesis and biological evaluation of Tc-99m radiolabeled AMO for miRNA imaging in tumor xenografts[J]. JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS,2015,58(13-14):461-468.
APA Kang, Lei.,Fan, Zhongyi.,Sun, Hongwei.,Feng, Yingying.,Ma, Chao.,...&Wang, Rongfu.(2015).Improved synthesis and biological evaluation of Tc-99m radiolabeled AMO for miRNA imaging in tumor xenografts.JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS,58(13-14),461-468.
MLA Kang, Lei,et al."Improved synthesis and biological evaluation of Tc-99m radiolabeled AMO for miRNA imaging in tumor xenografts".JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS 58.13-14(2015):461-468.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Kang, Lei]的文章
[Fan, Zhongyi]的文章
[Sun, Hongwei]的文章
百度学术
百度学术中相似的文章
[Kang, Lei]的文章
[Fan, Zhongyi]的文章
[Sun, Hongwei]的文章
必应学术
必应学术中相似的文章
[Kang, Lei]的文章
[Fan, Zhongyi]的文章
[Sun, Hongwei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。