IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
Receptor subtype involved in alpha(1)-adrenergic receptor-mediated Ca2+ signaling in cardiomyocytes
Luo, Da-li; Gao, Jian; Fan, Lin-lin; Tang, Yu; Zhang, You-yi; Han, Qi-de
关键词alpha(1)-adrenergic receptor subtype cardiomyocyte Ca2+ signaling A61603
刊名ACTA PHARMACOLOGICA SINICA
2007-07-01
DOI10.1111/j.1745-7254.2007.00605.x
28期:7页:968-974
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS ; RAT VENTRICULAR MYOCYTES ; ALPHA(1C)-ADRENERGIC RECEPTOR ; CARDIAC MYOCYTES ; ALPHA(1B)-ADRENERGIC RECEPTORS ; ALPHA(1A) SUBTYPE ; KNOCKOUT MOUSE ; MESSENGER-RNAS ; HYPERTROPHY ; HEARTS
英文摘要

Aim: The enhancement of intracellular Ca2+ signaling in response to alpha(1)-adrenergic receptor (alpha(1)-AR) stimulation is an essential signal transduction event in the regulation of cardiac functions, such as cardiac growth, cardiac contraction, and cardiac adaptation to various situations. The present study was intended to determine the role(s) of the alpha(1)-AR subtype(s) in mediating this response.

Methods: We evaluated the effects of subtype-specific agonists and antagonists of the alpha(1)-AR on the intracellular Ca2+ signaling of neonatal rat ventricular myocytes using a confocal microscope.

Results: After being cultured for 48 h, the myocytes exhibited spontaneous local Ca2+ release, sparks, and global Ca2+ transients. The activation of the alpha(1)-AR with phenylephrine, a selective agonist of the alpha(1)-AR, dose-dependently increased the frequency of Ca2+ transients with an EC50 value of 2.3 mu mol/L. Blocking the alpha(1A)-AR subtype with 5-methylurapidil (5-Mu) inhibited the stimulatory effect of phenylephrine with an IC50 value of 6.7 nmol/L. In contrast, blockade of the alpha(1B)-AR and alpha(1D)-AR subtypes with chlorethylclonidine and BMY 7378, respectively, did not affect the phenylephrine effect. Similarly, the local Ca2+ spark numbers were also increased by the activation of the alpha(1)-AR, and this effect could be abolished selectively by 5-Mu. More importantly, A61603, a novel selective alpha(1A)-AR agonist, mimicked the effect of phenylephrine, but with more potency (EC50 value=6.9 nmol/L) in the potentiation of Ca2+ transients, and blockade of the alpha(1A)-AR by 5-Mu caused abolishment of its effects.

Conclusion: These results indicate that alpha(1)-adrenergic stimulation of intracellular Ca2+ activity is mediated selectively by the alpha(1A)-AR.

语种英语
WOS记录号WOS:000247661400007
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65864
专题北京大学基础医学院_心血管所
作者单位1.Capital Univ Med Sci, Sch Chem Biol & Pharmaceut Sci, Dept Pharmacol, Beijing 100069, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Luo, Da-li,Gao, Jian,Fan, Lin-lin,et al. Receptor subtype involved in alpha(1)-adrenergic receptor-mediated Ca2+ signaling in cardiomyocytes[J]. ACTA PHARMACOLOGICA SINICA,2007,28(7):968-974.
APA Luo, Da-li,Gao, Jian,Fan, Lin-lin,Tang, Yu,Zhang, You-yi,&Han, Qi-de.(2007).Receptor subtype involved in alpha(1)-adrenergic receptor-mediated Ca2+ signaling in cardiomyocytes.ACTA PHARMACOLOGICA SINICA,28(7),968-974.
MLA Luo, Da-li,et al."Receptor subtype involved in alpha(1)-adrenergic receptor-mediated Ca2+ signaling in cardiomyocytes".ACTA PHARMACOLOGICA SINICA 28.7(2007):968-974.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
aps2007122a.pdf(1196KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Luo, Da-li]的文章
[Gao, Jian]的文章
[Fan, Lin-lin]的文章
百度学术
百度学术中相似的文章
[Luo, Da-li]的文章
[Gao, Jian]的文章
[Fan, Lin-lin]的文章
必应学术
必应学术中相似的文章
[Luo, Da-li]的文章
[Gao, Jian]的文章
[Fan, Lin-lin]的文章
相关权益政策
暂无数据
收藏/分享
文件名: aps2007122a.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。