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Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography
Ma, XF; Tu, PF; Chen, YJ; Zhang, TY; Wei, Y; Ito, Y
关键词Astragalus membranaceus counter-current chromatography plant materials preparative chromatography isoflavones calyposin glycoside formononetin glycoside glycosides
刊名JOURNAL OF CHROMATOGRAPHY A
2003-04-11
DOI10.1016/S0021-9673(03)00315-74
992期:1-2页:193-197
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SEPARATION ; FLAVONOIDS
英文摘要

Two isoflavones, calycosin-7-O-beta-D-glycoside and formononetin-7-O-beta-D-glycoside, were separated from n-butanol extract of the root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography in two steps using two different solvent systems composed of ethyl acetate-ethanol-n-butanol-water (30:10:6:50, v/v) and ethyl acetate-ethanol-water (5:1:5, v/v). From 200 mg of crude extract, calycosin-7-O-beta-D-glycoside (12 mg) and formononetin-7-O-beta-D-glycoside (10 mg) were isolated at over 95% purity by HPLC analyses, and their structures were identified by MS, H-1 NMR and C-13 NMR. (C) 2003 Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000182147600019
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被引频次:47[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
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条目标识符http://ir.bjmu.edu.cn/handle/400002259/65875
专题北京大学基础医学院_中西医结合学系
作者单位1.NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
2.Peking Univ, Modern Res Ctr Tradit Chinese Med, Beijing 100083, Peoples R China
3.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China
4.Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China
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Ma, XF,Tu, PF,Chen, YJ,et al. Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2003,992(1-2):193-197.
APA Ma, XF,Tu, PF,Chen, YJ,Zhang, TY,Wei, Y,&Ito, Y.(2003).Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,992(1-2),193-197.
MLA Ma, XF,et al."Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 992.1-2(2003):193-197.
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