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学科主题: 基础医学
题名:
Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography
作者: Ma, XF; Tu, PF; Chen, YJ; Zhang, TY; Wei, Y; Ito, Y
关键词: Astragalus membranaceus ; counter-current chromatography ; plant materials ; preparative chromatography ; isoflavones ; calyposin glycoside ; formononetin glycoside ; glycosides
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2003-04-11
DOI: 10.1016/S0021-9673(03)00315-74
卷: 992, 期:1-2, 页:193-197
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: SEPARATION ; FLAVONOIDS
英文摘要:

Two isoflavones, calycosin-7-O-beta-D-glycoside and formononetin-7-O-beta-D-glycoside, were separated from n-butanol extract of the root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography in two steps using two different solvent systems composed of ethyl acetate-ethanol-n-butanol-water (30:10:6:50, v/v) and ethyl acetate-ethanol-water (5:1:5, v/v). From 200 mg of crude extract, calycosin-7-O-beta-D-glycoside (12 mg) and formononetin-7-O-beta-D-glycoside (10 mg) were isolated at over 95% purity by HPLC analyses, and their structures were identified by MS, H-1 NMR and C-13 NMR. (C) 2003 Elsevier Science B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000182147600019
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65875
Appears in Collections:基础医学院_中西医结合学系_期刊论文

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作者单位: 1.NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
2.Peking Univ, Modern Res Ctr Tradit Chinese Med, Beijing 100083, Peoples R China
3.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China
4.Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China

Recommended Citation:
Ma, XF,Tu, PF,Chen, YJ,et al. Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2003,992(1-2):193-197.
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