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学科主题: 临床医学
题名:
Inhibition of CXCR4 expression by recombinant adenoviruses containing anti-sense RNA resists HIV-1 infection on MT4 cell lines
作者: Li, Wen-Gang1; Nie, Wei-Min1; Chen, Wei-Wei1; Jiang, Tian-Jun1; Xu, Xiao-Yuan1; Zhao, Min2
关键词: CXC chemokine receptor 4 ; Recombinant adenoviruses ; HIV-1 ; MT4 cell lines
刊名: GENE
发表日期: 2013-09-10
DOI: 10.1016/j.gene.2013.03.025
卷: 526, 期:2, 页:443-448
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
关键词[WOS]: CORECEPTOR ; RECEPTOR ; CCR5 ; THERAPY ; GENE ; REPLICATION ; MACROPHAGES ; PROGRESSION ; TROPISM ; CLONING
英文摘要:

CXCR4-tropic (X4) variants are associated with faster disease progression than CCR5-tropic variants in HIV infection. We previously reported inhibition of CCR5 expression on U937 cells could protect the cells from HIV-1 infection. Here, we established recombinant adenoviruses containing anti-sense CXCR4 cDNA, to investigate its role in the protection of HIV entering into target cells. A fragment of 636 bp cDNA from CXCR4 mRNA was recombined into adenoviral vector and the recombinant adenovirus was obtained from AD-293 cells. The rates of CXCR4 expression on the MT4 cells infected with recombinant adenovirus were measured by FACS. The MT4 cells infected by recombinant adenovirus were challenged by T-tropic HIV-1 strains and then P24 antigen was assayed. The rate of expression of CXCR4 on MT4 cell infected with recombinant adenovirus was decreased from 42% to 1.12% at 24 h, and to 1.03%, 1.39%, and 1.23% at 48 h, 72 h and 10 days respectively. Compared with Ad-control cells, recombinant adenovirus infected MT4 cells produced much less P24 antigen after being challenged with HIV-1. Furthermore, the recombinant adenovirus had no effects on chemotactic activity and proliferation of the MT4 cells. In conclusion, recombinant adenoviruses containing anti-sense can inhibit CXCR4 expression and resist HIV-1 infection on MT4 cell lines. (C) 2013 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30371266
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000323396500051
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65883
Appears in Collections:北京大学第一临床医学院_感染疾病科_期刊论文

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作者单位: 1.302 Hosp PLA, Treatment & Res Ctr Infect Dis, Beijing 100039, Peoples R China
2.Peking Univ First Hosp, Dept Infect Dis, Beijing 100034, Peoples R China

Recommended Citation:
Li, Wen-Gang,Nie, Wei-Min,Chen, Wei-Wei,et al. Inhibition of CXCR4 expression by recombinant adenoviruses containing anti-sense RNA resists HIV-1 infection on MT4 cell lines[J]. GENE,2013,526(2):443-448.
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