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学科主题基础医学
Molecular characterization of resistance, virulence and clonality in vancomycin-resistant Enterococcus faecium and Enterococcus faecalis: A hospital-based study in Beijing, China
Yang, Jing-xian1; Li, Tong2; Ning, Yong-zhong3; Shao, Dong-hua1; Liu, Jing1; Wang, Shu-qin1; Liang, Guo-wei1
关键词Vancomycin-resistant enterococcus Virulence Multilocus sequence typing
刊名INFECTION GENETICS AND EVOLUTION
2015-07-01
DOI10.1016/j.meegid.2015.05.012
33期:0页:253-260
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases
研究领域[WOS]Infectious Diseases
关键词[WOS]SEQUENCE TYPING SCHEME ; GLYCOPEPTIDE RESISTANCE ; MULTIPLEX PCR ; SPECIES LEVEL ; IDENTIFICATION ; EPIDEMIOLOGY ; COMPLEX ; SPREAD ; PREVALENCE ; BACTEREMIA
英文摘要

The incidence of vancomycin-resistant enterococcus (VRE) in China is increasing, the molecular epidemiology of VRE in China is only partly known. This study was conducted to assess the molecular characterization of resistance, virulence and clonality of 69 vancomycin-resistant Enterococcus faecium (VREfm) and seven vancomycin-resistant Enterococcus faecalis (VREfs) isolates obtained from a Chinese hospital between July 2011 and July 2013. The glycopeptide resistance genes (VanA and VanB) were screened by multiplex PCR. The presence of five putative virulence genes (esp, gelE, asa1, hyl and cylA) were evaluated by another multiplex PCR. Multilocus sequence typing (MLST) scheme was used to assess the clonality. All 76 VRE isolates exhibited VanA phenotype and harbored VanA gene. Esp was the only gene detected both in VREfm and VREfs strains, accounting for 89.9% and 42.9%, respectively. The hyl gene was merely positive in 27.5% of VREfm strains. MLST analysis demonstrated three STs (ST6, ST4 and ST470) in VREfs and twelve STs (ST78, ST571, ST17, ST564, ST389, ST18, ST547, ST341, ST414, ST343, ST262 and ST203) in VREfm, which were all designated as CC17 by eBURST algorithm. An outbreak of VREfm belonging to ST571 was found to happen within the neurology ward in this hospital. To our knowledge, this is the first report of ST6 (CC2) VREfs strains in China and the first outbreak report of VREfm strains belonging to ST571 around the world. Our data could offer important information for understanding the molecular features of VRE in China. (C) 2015 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000357438300035
资助机构Aerospace Center Hospital
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65898
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
北京大学第三临床医学院_检验科
作者单位1.Aerosp Ctr Hosp, Dept Clin Lab, Beijing 100049, Peoples R China
2.Peking Univ, Hosp 3, Dept Clin Lab, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
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Yang, Jing-xian,Li, Tong,Ning, Yong-zhong,et al. Molecular characterization of resistance, virulence and clonality in vancomycin-resistant Enterococcus faecium and Enterococcus faecalis: A hospital-based study in Beijing, China[J]. INFECTION GENETICS AND EVOLUTION,2015,33(0):253-260.
APA Yang, Jing-xian.,Li, Tong.,Ning, Yong-zhong.,Shao, Dong-hua.,Liu, Jing.,...&Liang, Guo-wei.(2015).Molecular characterization of resistance, virulence and clonality in vancomycin-resistant Enterococcus faecium and Enterococcus faecalis: A hospital-based study in Beijing, China.INFECTION GENETICS AND EVOLUTION,33(0),253-260.
MLA Yang, Jing-xian,et al."Molecular characterization of resistance, virulence and clonality in vancomycin-resistant Enterococcus faecium and Enterococcus faecalis: A hospital-based study in Beijing, China".INFECTION GENETICS AND EVOLUTION 33.0(2015):253-260.
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