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学科主题临床医学
The construction of an interfacial valve-based microfluidic chip for thermotaxis evaluation of human sperm
Li, Zhuoqi1,2; Liu, Weiran1,2; Qiu, Tian1,2; Xie, Lan1,2; Chen, Weixing1,2; Liu, Ran1; Lu, Ying1,2; Mitchelson, Keith1,2; Wang, Jundong3; Qiao, Jie4; Cheng, Jing1,2,5
刊名BIOMICROFLUIDICS
2014-03-01
DOI10.1063/1.4866851
8期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biophysics ; Nanoscience & Nanotechnology ; Physics, Fluids & Plasmas
资助者China&prime ; s National High-Tech Research Program ; China&prime ; s National High-Tech Research Program
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Science & Technology - Other Topics ; Physics
关键词[WOS]IN-VITRO FERTILIZATION ; MOTILE SPERM ; DEVICE ; SYSTEM ; POLY(DIMETHYLSILOXANE)
英文摘要

Thermotaxis has been demonstrated to be an important criterion for sperm evaluation, yet clinical assessment of thermotaxis capacity is currently lacking. In this article, the on-chip thermotaxis evaluation of human sperm is presented for the first time using an interfacial valve-facilitated microfluidic device. The temperature gradient was established and accurately controlled by an external temperature gradient control system. The temperature gradient responsive sperm population was enriched into one of the branch channels with higher temperature setting and the non-responsive ones were evenly distributed into the two branch channels. We employed air-liquid interfacial valves to ensure stable isolation of the two branches, facilitating convenient manipulation of the entrapped sperm. With this device, thermotactic responses were observed in 5.7%-10.6% of the motile sperm moving through four temperature ranges (34.0-35.3 degrees C, 35.0-36.3 degrees C, 36.0-37.3 degrees C, and 37.0-38.3 degrees C, respectively). In conclusion, we have developed a new method for high throughput clinical evaluation of sperm thermotaxis and this method may allow other researchers to derive better IVF procedure. (C) 2014 AIP Publishing LLC.

语种英语
所属项目编号2012AA020101
资助者China&prime ; s National High-Tech Research Program ; China&prime ; s National High-Tech Research Program
WOS记录号WOS:000336082100008
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65919
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Tsinghua Univ, Dept Biomed Engn, Sch Med, Beijing 100084, Peoples R China
2.Natl Engn Res Ctr Beijing Biochip Technol, Beijing 102206, Peoples R China
3.Shanxi Agr Univ, Shanxi Key Lab Ecol Anim Sci & Environm Med, Taigu 030801, Shanxi, Peoples R China
4.Peking Univ, Dept Obstet & Gynecol, Ctr Reprod Med, Hosp 3, Beijing 100083, Peoples R China
5.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
推荐引用方式
GB/T 7714
Li, Zhuoqi,Liu, Weiran,Qiu, Tian,et al. The construction of an interfacial valve-based microfluidic chip for thermotaxis evaluation of human sperm[J]. BIOMICROFLUIDICS,2014,8(2).
APA Li, Zhuoqi.,Liu, Weiran.,Qiu, Tian.,Xie, Lan.,Chen, Weixing.,...&Cheng, Jing.(2014).The construction of an interfacial valve-based microfluidic chip for thermotaxis evaluation of human sperm.BIOMICROFLUIDICS,8(2).
MLA Li, Zhuoqi,et al."The construction of an interfacial valve-based microfluidic chip for thermotaxis evaluation of human sperm".BIOMICROFLUIDICS 8.2(2014).
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