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学科主题: 基础医学
题名:
Long-Term In Vivo Imaging and Measurement of Dendritic Shrinkage of Retinal Ganglion Cells
作者: Leung, Christopher Kai-shun1; Weinreb, Robert N.3; Li, Zhi Wei1; Liu, Shu1; Lindsey, James D.3; Choi, Nathan1; Liu, Lan1; Cheung, Carol Yim-lui1; Ye, Cong1; Qiu, Kunliang1,4; Chen, Li Jia1; Yung, Wing Ho2; Crowston, Jonathan G.5; Pu, Mingliang6; So, Kwok Fai7; Pang, Chi Pui1; Lam, Dennis Shun Chiu1
刊名: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
发表日期: 2011-03-01
DOI: 10.1167/iovs.10-6012
卷: 52, 期:3, 页:1539-1547
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: SCANNING LASER OPHTHALMOSCOPY ; SPINAL-CORD ; LONGITUDINAL PROFILE ; AXONAL DEGENERATION ; MICE ; MOUSE ; REGENERATION ; DISEASE ; DAMAGE ; NERVE
英文摘要:

PURPOSE. To monitor and measure dendritic shrinkage of retinal ganglion cells (RGCs) in a strain of transgenic mice (Thy-1 YFP) that expresses yellow fluorescent proteins in neurons under the control of a Thy-1 promoter.

METHODS. A total of 125 RGCs from 16 eyes of Thy-1 YFP transgenic mice were serially imaged with a confocal scanning laser ophthalmoscope for 6 months after optic nerve crush. Quantitative analysis of cell body area, axon diameter, dendritic field, number of terminal branches, total dendritic branch length, branching complexity, symmetry, and distance from the optic disc was used to characterize the morphology of RGCs, describe the patterns of axonal and dendritic degeneration, identify the morphologic predictors for cell survival, and estimate the rate of dendritic shrinkage.

RESULTS. RGC damage was observed prospectively to begin with progressive dendritic shrinkage, followed by loss of the axon and the cell body. In a small proportion of RGCs, progressive axonal changes including fragmentation, beading, retraction, and bulb formation were also observed. RGCs with a larger dendritic field and a longer total dendritic branch length in general have a better survival probability. The rate of dendritic shrinkage was variable with a slower rate observed in cells having a larger dendritic field, a longer total dendritic branch length, and a greater distance from the optic disc.

CONCLUSIONS. Estimating the probability of RGC survival and measuring the rate of dendritic shrinkage could become a new paradigm for investigating neuronal degeneration and evaluating the response of neuroprotective treatment. (Invest Ophthalmol Vis Sci 2011;52:1539-1547) DOI:10.1167/iovs.10-6012

语种: 英语
项目资助者: CUHK ; Glaucoma Foundation
WOS记录号: WOS:000288965300041
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65923
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Univ Melbourne, Dept Ophthalmol, Melbourne, Vic, Australia
2.Chinese Univ Hong Kong, Dept Ophthalmol & Visual Sci, Kowloon, Hong Kong, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Kowloon, Hong Kong, Peoples R China
4.Univ Calif San Diego, Hamilton Glaucoma Ctr, San Diego, CA 92103 USA
5.Shantou Univ, Joint Shantou Int Eye Ctr, Kowloon, Hong Kong, Peoples R China
6.Peking Univ, Sch Basic Med Sci, Dept Anat & Embryol, Beijing 100871, Peoples R China
7.Univ Hong Kong, Dept Anat, State Key Lab Brain & Cognit Sci, Pokfulam, Hong Kong, Peoples R China

Recommended Citation:
Leung, Christopher Kai-shun,Weinreb, Robert N.,Li, Zhi Wei,et al. Long-Term In Vivo Imaging and Measurement of Dendritic Shrinkage of Retinal Ganglion Cells[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE,2011,52(3):1539-1547.
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