北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病原生物学系  > 期刊论文
学科主题: 基础医学
题名:
Dynamic changes in hepatitis C virus genotypes and sequence patterns in plasma donors exposed to reinfection
作者: Zhang, SM; Hui, Z; Li, HM; Qi, ZB; Widell, A
关键词: hepatitis C virus ; quasispecies ; nosocomial infection ; infected plasma donors ; mutation rate
刊名: JOURNAL OF MEDICAL VIROLOGY
发表日期: 2001-03-01
卷: 63, 期:3, 页:228-236
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Virology
研究领域[WOS]: Virology
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; HYPERVARIABLE REGION-1 ; SEXUAL TRANSMISSION ; LIVER-DISEASE ; RNA ; INFECTION ; PREVALENCE ; DIVERSITY ; ANTIBODY ; SWEDISH
英文摘要:

Sequential serum samples from four plasma donors (designated A, B, C, and D) at a Chinese blood bank with hepatitis C transmission problems were studied from 1994 to 1997. The samples were examined for antibodies to HCV, for HCV viremia by PCR and HCV genotyping. Co- and superinfections were studied by direct sequencing of the 5′-NCR, core, and HVR-1 regions, using low and high genotype-specific primers targeting the HVR-1, and by cloning of selected samples.

Genotype changes occurred in all four donors: A(1b-2a-1b), B(1b-2a-2a/1b-1b), C(1b-2a), and D (1b/2a-1b). Donor D was married to donor B. The 1b isolates of donor A could not be sequenced in the HVR-1 due to low-level viremia. Two early 1b isolates from donors B and C showed high HVR-1 similarity. The later 1b isolates from B had changed significantly but were identical to the isolate from donor D. Spouses B and D also shared genotype 2a strains. The 2a isolates from donors A, B/D, and C differed by 8-10 nucleotides in the HVR-1. The frequent changes in genotype and the appearance of homologous isolates from different subjects indicate transmission at the blood bank. These four donors, all identified shortly after infection, developed very few mutations in the HVR-1 and few quasispecies during a period of 6-18 months. Highly specific primers proved to be superior to cloning for identification of minor virus populations. The results indicate nosocomial transmission of more than one strain at the blood bank studied. J. Med. Virol. 63: 228-236, 2001. (C) 2001 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000166755100006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65948
Appears in Collections:基础医学院_病原生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Dynamic changes in hepatitis C virus genotypes and sequence patterns in plasma donors exposed to reinfection.pdf(128KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Univ Lund, Malmo Univ Hosp, Dept Med Microbiol, S-20502 Malmo, Sweden
2.Natl Inst Control Pharmaceut & Biol Prod, Beijing 100050, Peoples R China
3.Beijing Med Univ, Dept Microbiol, Beijing 100087, Peoples R China

Recommended Citation:
Zhang, SM,Hui, Z,Li, HM,et al. Dynamic changes in hepatitis C virus genotypes and sequence patterns in plasma donors exposed to reinfection[J]. JOURNAL OF MEDICAL VIROLOGY,2001,63(3):228-236.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, SM]'s Articles
[Hui, Z]'s Articles
[Li, HM]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, SM]‘s Articles
[Hui, Z]‘s Articles
[Li, HM]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace