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Simultaneous determination of five aristolochic acids and two aristololactams in Aristolochia plants by high-performance liquid chromatography
Zhang, CY; Wang, X; Shang, MY; Yu, J; Xu, YQ; Li, ZG; Lei, LC; Li, XM; Cai, SQ; Namba, T
关键词Aristolochia aristolochic acid aristololactam determination HPLC
刊名BIOMEDICAL CHROMATOGRAPHY
2006-04-01
DOI10.1002/bmc.565
20期:4页:309-318
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]CAPILLARY-ZONE-ELECTROPHORESIS ; MASS-SPECTROMETRY ; MEDICINAL-PLANTS ; CHINESE HERBS ; CONSTITUENTS ; NEPHROPATHY ; FIBROSIS
英文摘要

An HPLC method was developed for the simultaneous determination of five aristolochic acids (AAs) and two aristololactams (ALs) in the following six Chinese drugs derived from Aristolochia species. Samples were analyzed on a C-18 column with acetonitrile and 3.7 m(M) phosphoric acid buffer gradient elution, detected at 260 nm. Assay was linear over the range (mu g/mL) 0.386-38.6 for aristolochic acid Va, 0.632-63.2 for aristolochic acid IVa, 0.200-20.0 for 9-hydroxy aristolochic acid 1, 0.352-35.2 for aristololactam II, 0.296-29.6 for aristolochic acid II, 0.274-27.4 for aristololactam I and 3.12-312 for aristolochic acid I. Average recoveries (%) of samples were 102.0, 95.9, 99.2, 102.2, 97.2. 97.1 and 97.8 for these seven constituents, respectively. The detection limit and retention time for the seven constituents ranged from 10.0 to 15.8 ng/mL and from 12 to 21 min. As a result of drug determination, contents (in mg/g) were as follows: AA-I. 0.69-1.77; AA-II, 0.02-0.18: 9-OH AA-I, 0.04-0.12; AA-IVa, 0.76-3.36; AA-Va, 0.04-0.31: AL-I, 0.07-0.36; and AL-II, 0.01-0.09 in Madouling; AA-I, 0.03-0.41; AA-II, 0.01-0.11; 9-OH AA-I, 0.00-0.60; AA-IVa, 0.00-0.77; AA-Va, 0.00-0.14; and AL-I, 0.00-0.04 in Tianxianteng; AA-I, 1.19-4.71; and AA-II, 0.24-1.69 in Qingmuxiang; AA-I, 2.79-5.48; AA-II, 1.06-1.86, 9-OH AA-I, 0.01-0.09: AA-IVa, 0.38-0.69, AA-Va, 0.00-0.61; AL-I, 0.00-0.02; and AL-II, 0.00-0.02 in Bei-madouling-gen; AA-I, 0.64-4.23; AA-II, 0.06-0.40, and AA-IVa, 0.08-0.25; in Guangfangji; and AA-I, 1.88-9.72; AA-II, 0.26-1.88; and AA-IVa, 0.09-0.52 in Guanmutong. The other constituents were not detected in Tianxianteng, Qingmuxiang, Guangfangji and Guanmutong. Copyright (c) 2005 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000237238200002
引用统计
被引频次:42[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65956
专题北京大学药学院_天然药物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
2.Henan Inst Drug Control, Zhengzhou, Peoples R China
3.Toyama Med & Pharmaceut Univ, Sugitani, Toyama 93001, Japan
4.Peking Univ, Hosp 1, Dept Med, Div Renal, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, CY,Wang, X,Shang, MY,et al. Simultaneous determination of five aristolochic acids and two aristololactams in Aristolochia plants by high-performance liquid chromatography[J]. BIOMEDICAL CHROMATOGRAPHY,2006,20(4):309-318.
APA Zhang, CY.,Wang, X.,Shang, MY.,Yu, J.,Xu, YQ.,...&Namba, T.(2006).Simultaneous determination of five aristolochic acids and two aristololactams in Aristolochia plants by high-performance liquid chromatography.BIOMEDICAL CHROMATOGRAPHY,20(4),309-318.
MLA Zhang, CY,et al."Simultaneous determination of five aristolochic acids and two aristololactams in Aristolochia plants by high-performance liquid chromatography".BIOMEDICAL CHROMATOGRAPHY 20.4(2006):309-318.
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