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学科主题: 药学
题名:
Simultaneous determination of five aristolochic acids and two aristololactams in Aristolochia plants by high-performance liquid chromatography
作者: Zhang, CY; Wang, X; Shang, MY; Yu, J; Xu, YQ; Li, ZG; Lei, LC; Li, XM; Cai, SQ; Namba, T
关键词: Aristolochia ; aristolochic acid ; aristololactam ; determination ; HPLC
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2006-04-01
DOI: 10.1002/bmc.565
卷: 20, 期:4, 页:309-318
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: CAPILLARY-ZONE-ELECTROPHORESIS ; MASS-SPECTROMETRY ; MEDICINAL-PLANTS ; CHINESE HERBS ; CONSTITUENTS ; NEPHROPATHY ; FIBROSIS
英文摘要:

An HPLC method was developed for the simultaneous determination of five aristolochic acids (AAs) and two aristololactams (ALs) in the following six Chinese drugs derived from Aristolochia species. Samples were analyzed on a C-18 column with acetonitrile and 3.7 m(M) phosphoric acid buffer gradient elution, detected at 260 nm. Assay was linear over the range (mu g/mL) 0.386-38.6 for aristolochic acid Va, 0.632-63.2 for aristolochic acid IVa, 0.200-20.0 for 9-hydroxy aristolochic acid 1, 0.352-35.2 for aristololactam II, 0.296-29.6 for aristolochic acid II, 0.274-27.4 for aristololactam I and 3.12-312 for aristolochic acid I. Average recoveries (%) of samples were 102.0, 95.9, 99.2, 102.2, 97.2. 97.1 and 97.8 for these seven constituents, respectively. The detection limit and retention time for the seven constituents ranged from 10.0 to 15.8 ng/mL and from 12 to 21 min. As a result of drug determination, contents (in mg/g) were as follows: AA-I. 0.69-1.77; AA-II, 0.02-0.18: 9-OH AA-I, 0.04-0.12; AA-IVa, 0.76-3.36; AA-Va, 0.04-0.31: AL-I, 0.07-0.36; and AL-II, 0.01-0.09 in Madouling; AA-I, 0.03-0.41; AA-II, 0.01-0.11; 9-OH AA-I, 0.00-0.60; AA-IVa, 0.00-0.77; AA-Va, 0.00-0.14; and AL-I, 0.00-0.04 in Tianxianteng; AA-I, 1.19-4.71; and AA-II, 0.24-1.69 in Qingmuxiang; AA-I, 2.79-5.48; AA-II, 1.06-1.86, 9-OH AA-I, 0.01-0.09: AA-IVa, 0.38-0.69, AA-Va, 0.00-0.61; AL-I, 0.00-0.02; and AL-II, 0.00-0.02 in Bei-madouling-gen; AA-I, 0.64-4.23; AA-II, 0.06-0.40, and AA-IVa, 0.08-0.25; in Guangfangji; and AA-I, 1.88-9.72; AA-II, 0.26-1.88; and AA-IVa, 0.09-0.52 in Guanmutong. The other constituents were not detected in Tianxianteng, Qingmuxiang, Guangfangji and Guanmutong. Copyright (c) 2005 John Wiley & Sons, Ltd.

语种: 英语
WOS记录号: WOS:000237238200002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65956
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
2.Henan Inst Drug Control, Zhengzhou, Peoples R China
3.Toyama Med & Pharmaceut Univ, Sugitani, Toyama 93001, Japan
4.Peking Univ, Hosp 1, Dept Med, Div Renal, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, CY,Wang, X,Shang, MY,et al. Simultaneous determination of five aristolochic acids and two aristololactams in Aristolochia plants by high-performance liquid chromatography[J]. BIOMEDICAL CHROMATOGRAPHY,2006,20(4):309-318.
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