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IR@PKUHSC  > 北京大学第二临床医学院  > 北京大学肝病研究所  > 期刊论文
学科主题: 临床医学
题名:
Effects of IL-1 beta on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells
作者: Guo, Tao1; Qian, Jia-Ming1; Zhao, Yu-Qing1; Li, Xiao-Bo2; Zhang, Jian-Zhong3
关键词: Helicobacter pylori ; gastric cancer ; interleukin-1 ; cyclooxygenase-2 ; H+/K(+)ATPase
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2013
DOI: 10.3892/mmr.2012.1165
卷: 7, 期:1, 页:299-305
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: HELICOBACTER-PYLORI INFECTION ; HUMAN COLORECTAL-CANCER ; CYCLOOXYGENASE 2 ; PGE(2) RELEASE ; EXPRESSION ; COX-2 ; ACTIVATION ; POLYMORPHISMS ; INTERLEUKIN-1-BETA ; SUPPRESSION
英文摘要:

The aim of the present study was to explore the effects of IL-1 beta on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells. The mechanisms by which these effects are mediated were also investigated. Parietal cells were isolated from rabbit gastric mucosa by elutriation. The AGS human gastric cancer cell line, the GES-1 human gastric epithelial cell line and parietal cells were treated with interleukin (IL)-1 beta in the presence or absence of Helicobacter pylori (H. pylori) for the times indicated. MTT assay and flow cytometry (FCM) were used to determine the levels of proliferation and apoptosis. The expression levels of cyclooxygenase-2 (COX-2) mRNA and protein were examined by RT-PCR and FCM. Acid secretion by parietal cells was examined using C-14-aminopyrine (C-14-AP) accumulation. H+/K(+)ATPase alpha subunit mRNA expression was assessed by RT-PCR. The results demonstrated that IL-1 beta (10 ng/ml) stimulated cellular proliferation and inhibited H. pylori-induced apoptosis in GES-1 and AGS cell lines. IL-1 beta (10 ng/ml) upregulated the mRNA and protein expression of COX-2 in GES-1 and AGS cells. Acid secretion stimulated by histamine was identified as significantly inhibited and mRNA expression of H+/K(+)ATPase alpha subunit was downregulated by treatment with IL-1 beta (10 ng/ml) for 30 min and 16 h compared with the control in isolated rabbit parietal cells. The present study demonstrates that IL-1 beta plays a significant role in H. pylori-induced gastric carcinogenesis through 2 main mechanisms: i) IL-1 beta may interfere in gastric epithelial cell growth by upregulating COX-2 expression; ii) IL-1 beta may inhibit the acid secretion from parietal cells by downregulating H+/K(+)ATPase expression.

语种: 英语
所属项目编号: NSFC-30270600
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000312238000053
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65978
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Dept Gastroenterol, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
2.Beijing Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
3.Chinese Ctr Dis Control & Prevent, Inst Communicable Dis Control & Prevent, Dept Diag, Beijing 102206, Peoples R China

Recommended Citation:
Guo, Tao,Qian, Jia-Ming,Zhao, Yu-Qing,et al. Effects of IL-1 beta on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells[J]. MOLECULAR MEDICINE REPORTS,2013,7(1):299-305.
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