学科主题临床医学
Effects of IL-1 beta on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells
Guo, Tao1; Qian, Jia-Ming1; Zhao, Yu-Qing1; Li, Xiao-Bo2; Zhang, Jian-Zhong3
关键词Helicobacter pylori gastric cancer interleukin-1 cyclooxygenase-2 H+/K(+)ATPase
刊名MOLECULAR MEDICINE REPORTS
2013
DOI10.3892/mmr.2012.1165
7期:1页:299-305
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]HELICOBACTER-PYLORI INFECTION ; HUMAN COLORECTAL-CANCER ; CYCLOOXYGENASE 2 ; PGE(2) RELEASE ; EXPRESSION ; COX-2 ; ACTIVATION ; POLYMORPHISMS ; INTERLEUKIN-1-BETA ; SUPPRESSION
英文摘要

The aim of the present study was to explore the effects of IL-1 beta on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells. The mechanisms by which these effects are mediated were also investigated. Parietal cells were isolated from rabbit gastric mucosa by elutriation. The AGS human gastric cancer cell line, the GES-1 human gastric epithelial cell line and parietal cells were treated with interleukin (IL)-1 beta in the presence or absence of Helicobacter pylori (H. pylori) for the times indicated. MTT assay and flow cytometry (FCM) were used to determine the levels of proliferation and apoptosis. The expression levels of cyclooxygenase-2 (COX-2) mRNA and protein were examined by RT-PCR and FCM. Acid secretion by parietal cells was examined using C-14-aminopyrine (C-14-AP) accumulation. H+/K(+)ATPase alpha subunit mRNA expression was assessed by RT-PCR. The results demonstrated that IL-1 beta (10 ng/ml) stimulated cellular proliferation and inhibited H. pylori-induced apoptosis in GES-1 and AGS cell lines. IL-1 beta (10 ng/ml) upregulated the mRNA and protein expression of COX-2 in GES-1 and AGS cells. Acid secretion stimulated by histamine was identified as significantly inhibited and mRNA expression of H+/K(+)ATPase alpha subunit was downregulated by treatment with IL-1 beta (10 ng/ml) for 30 min and 16 h compared with the control in isolated rabbit parietal cells. The present study demonstrates that IL-1 beta plays a significant role in H. pylori-induced gastric carcinogenesis through 2 main mechanisms: i) IL-1 beta may interfere in gastric epithelial cell growth by upregulating COX-2 expression; ii) IL-1 beta may inhibit the acid secretion from parietal cells by downregulating H+/K(+)ATPase expression.

语种英语
WOS记录号WOS:000312238000053
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/65978
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Chinese Acad Med Sci, Dept Gastroenterol, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
2.Beijing Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
3.Chinese Ctr Dis Control & Prevent, Inst Communicable Dis Control & Prevent, Dept Diag, Beijing 102206, Peoples R China
推荐引用方式
GB/T 7714
Guo, Tao,Qian, Jia-Ming,Zhao, Yu-Qing,et al. Effects of IL-1 beta on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells[J]. MOLECULAR MEDICINE REPORTS,2013,7(1):299-305.
APA Guo, Tao,Qian, Jia-Ming,Zhao, Yu-Qing,Li, Xiao-Bo,&Zhang, Jian-Zhong.(2013).Effects of IL-1 beta on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells.MOLECULAR MEDICINE REPORTS,7(1),299-305.
MLA Guo, Tao,et al."Effects of IL-1 beta on the proliferation and apoptosis of gastric epithelial cells and acid secretion from isolated rabbit parietal cells".MOLECULAR MEDICINE REPORTS 7.1(2013):299-305.
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