北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 血液内科  > 期刊论文
学科主题: 临床医学
题名:
An improved design of PCR primers for detection of human T cell receptor beta chain repertoire
作者: Ou, Yuan1; Tong, Chunrong2; Zhang, Ying1; Cai, Peng2; Gu, Jiangying2; Liu, Yan2; Liu, Hongxing2; Wang, He2; Chu, Bin1; Zhu, Ping1,2
关键词: T cell receptor ; Primers ; Variable region ; T cells ; beta chain
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2009
DOI: 10.1007/s11033-008-9328-y
卷: 36, 期:1, 页:145-152
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: DIVERSITY ; IMMUNODEFICIENCY ; TRANSPLANTATION ; SUPERANTIGENS ; EXPRESSION ; RESPONSES ; PATTERNS
英文摘要:

Analysis of T cell receptor beta variable region (TCRBV) gene rearrangement is useful for clonal assessment of abnormal T cell proliferations in various diseases. However, most primer panels previously used can only amplify the third complementarity-determining region. Following IMGT database we established a panel of primers, which can amplify entire sequences of all functional TCRBV families. To confirm the usefulness of this panel of primers, we detected different TCRBV repertoires. In 15 healthy donors, most of the BV families were expressed and appeared as a Gaussian distribution. 13 acute myeloid leukemia patients showed monoclonal or oligoclonal changes of BV15 family, and some of them also had monoclonal or oligoclonal expansion of BV2, BV4, BV6 or BV13 families. In one patient after allo-hematopoietic stem cell transplantation, monoclonal proliferation of BV10 family occurred during graft-versus-host disease. In conclusion, this panel of primers improves our abilities to analyze TCRBV repertoire changes in related diseases.

语种: 英语
所属项目编号: 30470939 ; 2006DFB31430
项目资助者: China National Natural Science Foundation ; China National Ministry of Science and Technology
WOS记录号: WOS:000262088100020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/65990
Appears in Collections:北京大学第一临床医学院_血液内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 1, Ctr Med Genet, Dept Hematol Oncol, Beijing 100034, Peoples R China
2.Beijing Daopei Hosp, Beijing 100039, Peoples R China

Recommended Citation:
Ou, Yuan,Tong, Chunrong,Zhang, Ying,et al. An improved design of PCR primers for detection of human T cell receptor beta chain repertoire[J]. MOLECULAR BIOLOGY REPORTS,2009,36(1):145-152.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ou, Yuan]'s Articles
[Tong, Chunrong]'s Articles
[Zhang, Ying]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ou, Yuan]‘s Articles
[Tong, Chunrong]‘s Articles
[Zhang, Ying]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace