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学科主题: 临床医学
题名:
FAP-alpha (Fibroblast activation protein-alpha) is involved in the control of human breast cancer cell line growth and motility via the FAK pathway
作者: Jia, Jun1,2; Martin, Tracey Amanda1; Ye, Lin1; Jiang, Wen Guo1
关键词: FAP-alpha ; FAK ; Breast Cancer ; Growth ; Migration
刊名: BMC CELL BIOLOGY
发表日期: 2014-05-21
DOI: 10.1186/1471-2121-15-16
卷: 15
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: DIFFERENT EXPRESSION PATTERNS ; INVASIVE DUCTAL CARCINOMA ; MEMBRANE-BOUND PROTEASE ; SERINE-PROTEASE ; TUMOR-GROWTH ; COLON-CANCER ; COLORECTAL-CANCER ; SEPRASE ; SUPPRESSION ; ADHESION
英文摘要:

Background: Fibroblast Activation Protein alpha (FAP-alpha) or seprase is an integral membrane serine peptidase. Previous work has not satisfactorily explained both the suppression and promotion effects that have been observed in cancer. The purpose of this work was to investigate the role of FAP-alpha in human breast cancer. Expression of FAP-alpha was characterized in primary tumour samples and in cell lines, along with the effects of FAP-alpha expression on in vitro growth, invasion, attachment and migration. Furthermore the potential interaction of FAP-alpha with other signalling pathways was investigated.

Results: FAP-alpha was significantly increased in patients with poor outcome and survival. In vitro results showed that breast cancer cells over expressing FAP-alpha had increased growth ability and impaired migratory ability. The growth of MDA-MB-231 cells and the adhesion and invasion ability of both MCF-7 cells and MDA-MB-231 cells were not dramatically influenced by FAP-alpha expression. Over-expression of FAP-alpha resulted in a reduction of phosphorylated focal adhesion kinase (FAK) level in both cells cultured in normal media and serum-free media. An inhibitor to FAK restored the reduced motility ability of both MCF-7exp cells and MDA-MB-231exp cells and prevented the change in phosphorylated FAK levels. However, inhibitors to PI3K, ERK, PLC gamma, NWASP, ARP2/3, and ROCK had no influence this.

Conclusions: FAP-alpha in significantly associated with poor outcome in patients with breast cancer. In vitro, FAP-alpha promotes proliferation and inhibits migration of breast cancer cells, potentially by regulating the FAK pathway. These results suggest FAP-alpha could be a target for future therapies.

语种: 英语
项目资助者: Cancer Research Wales ; Albert Hong Foundation ; Breast Cancer Hope Foundation
WOS记录号: WOS:000338150500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66003
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Cardiff Univ, Sch Med, Inst Canc, Peking Univ, Cardiff CF14 4XN, S Glam, Wales
2.Peking Univ, Sch Oncol, Beijing Canc Hosp, Beijing 100142, Peoples R China

Recommended Citation:
Jia, Jun,Martin, Tracey Amanda,Ye, Lin,et al. FAP-alpha (Fibroblast activation protein-alpha) is involved in the control of human breast cancer cell line growth and motility via the FAK pathway[J]. BMC CELL BIOLOGY,2014,15.
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