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Engineering Bacterial Cellulose with Bone Marrow Stem Cells and Meniscal Fibrochondrocytes for Meniscal Repair
Chen, Guofei1; Zhang, Honglei2; Bai, Lu2; Jiang, Changqing2; Zhang, Xintao2; Huang, Wei2; Zhang, Wentao1,2
关键词Bacterial Cellulose Meniscal Repair Meniscal Fibrochondrocyte Bone Marrow Mesenchymal Stem Cell Biocompatibility Inflammation
刊名JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
2015-02-01
DOI10.1166/jbt.2015.1293
5期:2页:92-98
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering
研究领域[WOS]Cell Biology
关键词[WOS]TISSUE ; BIOCOMPATIBILITY ; SCAFFOLDS ; BIODEGRADABILITY ; PROLIFERATION ; CHONDROCYTES
英文摘要

Meniscal tears are the most common knee injuries caused by a rupture of one or more of the fibrocartilage strips in the knee, which causes pain in the joint. Partial or complete meniscal replacement with engineered materials has gained increasing attention. This study tries to characterize a bacterial cellulose (BC) for potential use in meniscal repair. The toxicity of BC was assayed by MTT after cells were cultured with BC extraction medium. Cell growth on BC sheets and in vitro differentiation of bone marrow stem cells (BMSCs) into meniscal fibrochondrocytes (MFCs) were analyzed by HE staining, quantitative RT-PCR, ICC, and scanning electron microscopy. The serum IL-6 and TNF-alpha levels were measured by ELISA, macrophage infiltration by CD68 IHC, and tissue repair by HE staining. When co-cultured with MFCs, BMSCs were induced to differentiate into MFCs. The extraction medium of BC sheets did not inhibit MFC growth. Implantation of MFC and BMSC-engineered BC sheets into rabbit buttock muscles did not elicit inflammatory reactions and supported injury recovery. The bacterial cellulose exhibited great potential to be engineered for meniscal repair due to its biocompatability, non-immunoactivity, and bioactivity to support BMSC and MFC growth and differentiation.

语种英语
WOS记录号WOS:000354710900002
项目编号4120124
资助机构Shenzhen Technical Innovation Committee
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66007
专题北京大学深圳医院
作者单位1.Anhui Med Univ, Dept Sports Med, Hefei 230032, Anhui, Peoples R China
2.Peking Univ, Shenzhen Hosp, Clin Sch, Shenzhen 518000, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Chen, Guofei,Zhang, Honglei,Bai, Lu,et al. Engineering Bacterial Cellulose with Bone Marrow Stem Cells and Meniscal Fibrochondrocytes for Meniscal Repair[J]. JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,2015,5(2):92-98.
APA Chen, Guofei.,Zhang, Honglei.,Bai, Lu.,Jiang, Changqing.,Zhang, Xintao.,...&Zhang, Wentao.(2015).Engineering Bacterial Cellulose with Bone Marrow Stem Cells and Meniscal Fibrochondrocytes for Meniscal Repair.JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,5(2),92-98.
MLA Chen, Guofei,et al."Engineering Bacterial Cellulose with Bone Marrow Stem Cells and Meniscal Fibrochondrocytes for Meniscal Repair".JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING 5.2(2015):92-98.
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