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学科主题: 临床医学
题名:
Engineering Bacterial Cellulose with Bone Marrow Stem Cells and Meniscal Fibrochondrocytes for Meniscal Repair
作者: Chen, Guofei1; Zhang, Honglei2; Bai, Lu2; Jiang, Changqing2; Zhang, Xintao2; Huang, Wei2; Zhang, Wentao1,2
关键词: Bacterial Cellulose ; Meniscal Repair ; Meniscal Fibrochondrocyte ; Bone Marrow Mesenchymal Stem Cell ; Biocompatibility ; Inflammation
刊名: JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING
发表日期: 2015-02-01
DOI: 10.1166/jbt.2015.1293
卷: 5, 期:2, 页:92-98
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering
研究领域[WOS]: Cell Biology
关键词[WOS]: TISSUE ; BIOCOMPATIBILITY ; SCAFFOLDS ; BIODEGRADABILITY ; PROLIFERATION ; CHONDROCYTES
英文摘要:

Meniscal tears are the most common knee injuries caused by a rupture of one or more of the fibrocartilage strips in the knee, which causes pain in the joint. Partial or complete meniscal replacement with engineered materials has gained increasing attention. This study tries to characterize a bacterial cellulose (BC) for potential use in meniscal repair. The toxicity of BC was assayed by MTT after cells were cultured with BC extraction medium. Cell growth on BC sheets and in vitro differentiation of bone marrow stem cells (BMSCs) into meniscal fibrochondrocytes (MFCs) were analyzed by HE staining, quantitative RT-PCR, ICC, and scanning electron microscopy. The serum IL-6 and TNF-alpha levels were measured by ELISA, macrophage infiltration by CD68 IHC, and tissue repair by HE staining. When co-cultured with MFCs, BMSCs were induced to differentiate into MFCs. The extraction medium of BC sheets did not inhibit MFC growth. Implantation of MFC and BMSC-engineered BC sheets into rabbit buttock muscles did not elicit inflammatory reactions and supported injury recovery. The bacterial cellulose exhibited great potential to be engineered for meniscal repair due to its biocompatability, non-immunoactivity, and bioactivity to support BMSC and MFC growth and differentiation.

语种: 英语
所属项目编号: 4120124
项目资助者: Shenzhen Technical Innovation Committee
WOS记录号: WOS:000354710900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66007
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Anhui Med Univ, Dept Sports Med, Hefei 230032, Anhui, Peoples R China
2.Peking Univ, Shenzhen Hosp, Clin Sch, Shenzhen 518000, Guangdong, Peoples R China

Recommended Citation:
Chen, Guofei,Zhang, Honglei,Bai, Lu,et al. Engineering Bacterial Cellulose with Bone Marrow Stem Cells and Meniscal Fibrochondrocytes for Meniscal Repair[J]. JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING,2015,5(2):92-98.
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