|Myeloid-derived suppressor cells have a proinflammatory role in the pathogenesis of autoimmune arthritis|
|Guo, Chunqing1,2,3; Hu, Fanlei4; Yi, Huanfa1,2,3,5; Feng, Zhitao6,7; Li, Changzheng6,7; Shi, Lianjie4; Li, Yingni4; Liu, Hongjiang4; Yu, Xiaofei1,2,3; Wang, Hongxia1,2,3; Li, Juan6,7; Li, Zhanguo4; Wang, Xiang-Yang1,2,3|
|刊名||ANNALS OF THE RHEUMATIC DISEASES|
|WOS标题词||Science & Technology|
|关键词[WOS]||COLLAGEN-INDUCED ARTHRITIS ; TH17 CELLS ; RHEUMATOID-ARTHRITIS ; T-CELLS ; IMMUNE-SYSTEM ; DISEASE ; INTERLEUKIN-17 ; MICE ; INDUCTION ; PLAY|
Objectives Although myeloid-derived suppressor cells (MDSCs) have been linked to T cell tolerance, their role in autoimmune rheumatoid arthritis (RA) remains elusive. Here we investigate the potential association of MDSCs with the disease pathogenesis using a preclinical model of RA and specimen collected from patients with RA.
Methods The frequency of MDSCs in blood, lymphoid tissues, inflamed paws or synovial fluid and their association with disease severity, tissue inflammation and the levels of pathogenic T helper (Th) 17 cells were examined in arthritic mice or in patients with RA (n=35) and osteoarthritis (n=15). The MDSCs in arthritic mice were also characterised for their phenotype, inflammation status, T cell suppressive activity and their capacity of pro-Th17 cell differentiation. The involvement of MDSCs in the disease pathology and a Th17 response was examined by adoptive transfer or antibody depletion of MDSCs in arthritic mice or by coculturing mouse or human MDSCs with naive CD4+T cells under Th17-polarising conditions.
Results MDSCs significantly expanded in arthritic mice and in patients with RA, which correlated positively with disease severity and an inflammatory Th17 response. While displaying T cell suppressive activity, MDSCs from arthritic mice produced high levels of inflammatory cytokines (eg, interleukin (IL)-1 beta, TNF-alpha). Mouse and human MDSCs promoted Th17 cell polarisation ex vivo. Transfer of MDSCs facilitated disease progression, whereas their elimination in arthritic mice ameliorated disease symptoms concomitant with reduction of IL-17A/Th17 cells.
Conclusions Our studies suggest that proinflammatory MDSCs with their capacity to drive Th17 cell differentiation may be a critical pathogenic factor in autoimmune arthritis.
|项目编号||CA175033 ; CA154708 ; 81302554 ; 81030057 ; 81173456 ; 2010CB529100 ; RDB2013-04 ; P30CA16059|
|资助机构||National Institutes of Health (NIH) ; Natural Science Foundation of China ; 973 Program of China ; Peking University People&prime ; s Hospital Research and Development Fund ; NCI Cancer Center Support Grant|
|作者单位||1.Virginia Commonwealth Univ, Sch Med, Dept Human & Mol Genet, Richmond, VA 23298 USA|
2.Virginia Commonwealth Univ, Sch Med, Inst Mol Med, Richmond, VA 23298 USA
3.Virginia Commonwealth Univ, Sch Med, Massey Canc Ctr, Richmond, VA 23298 USA
4.Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100871, Peoples R China
5.Jilin Univ, Inst Immunol, Bethune Hosp 1, Changchun 130023, Jilin, Peoples R China
6.Southern Med Univ, Nanfang Hosp, Dept Rheumatol, Guangzhou, Guangdong, Peoples R China
7.Southern Med Univ, Sch Tradit Chinese Med, Dept Tradit Chinese Internal Med, Guangzhou, Guangdong, Peoples R China
|Guo, Chunqing,Hu, Fanlei,Yi, Huanfa,et al. Myeloid-derived suppressor cells have a proinflammatory role in the pathogenesis of autoimmune arthritis[J]. ANNALS OF THE RHEUMATIC DISEASES,2016,75(1):278-285.|
|APA||Guo, Chunqing.,Hu, Fanlei.,Yi, Huanfa.,Feng, Zhitao.,Li, Changzheng.,...&Wang, Xiang-Yang.(2016).Myeloid-derived suppressor cells have a proinflammatory role in the pathogenesis of autoimmune arthritis.ANNALS OF THE RHEUMATIC DISEASES,75(1),278-285.|
|MLA||Guo, Chunqing,et al."Myeloid-derived suppressor cells have a proinflammatory role in the pathogenesis of autoimmune arthritis".ANNALS OF THE RHEUMATIC DISEASES 75.1(2016):278-285.|