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Caged circular antisense oligonucleotides for photomodulation of RNA digestion and gene expression in cells
Wu, Li; Wang, Yuan; Wu, Junzhou; Lv, Cong; Wang, Jie; Tang, Xinjing
刊名NUCLEIC ACIDS RESEARCH
2013
DOI10.1093/nar/gks996
41期:1页:677-686
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]LIGHT-ACTIVATED OLIGONUCLEOTIDES ; ZEBRAFISH EMBRYOS ; MAMMALIAN-CELLS ; DNA ; PHOTOREGULATION ; HYBRIDIZATION ; TRANSCRIPTION ; INTERFERENCE ; AZOBENZENE ; CLEAVAGE
英文摘要

We synthesized three 20mer caged circular antisense oligodeoxynucleotides (R20, R20B2 and R20B4) with a photocleavable linker and an amide bond linker between two 10mer oligodeoxynucleotides. With these caged circular antisense oligodeoxynucleotides, RNA-binding affinity and its digestion by ribonuclease H were readily photomodulated. RNA cleavage rates were upregulated similar to 43-, 25- and 15-fold for R20, R20B2 and R20B4, respectively, upon light activation in vitro. R20B2 and R20B4 with 2- or 4-nt gaps in the target RNA lost their ability to bind the target RNA even though a small amount of RNA digestion was still observed. The loss of binding ability indicated promising gene photoregulation through a non-enzymatic strategy. To test this strategy, three caged circular antisense oligonucleotides (PS1, PS2 and PS3) with 2′-OMe RNA and phosphorothioate modifications were synthesized to target GFP expression. Upon light activation, photomodulation of target hybridization and GFP expression in cells was successfully achieved with PS1, PS2 and PS3. These caged circular antisense oligonucleotides show promising applications of photomodulating gene expression through both ribonuclease H and non-enzyme involved antisense strategies.

语种英语
WOS记录号WOS:000312889900088
项目编号21072015 ; 2012CB720600 ; NCET-10-0203 ; 2012M510290 ; 21072015 ; 2012M510290
资助机构National Science Foundation of China (NSFC) ; National Basic Research Program of China [&prime ; 973&prime ; Program] ; Program for New Century Excellent Talents in University ; China Postdoctoral Science Foundation (CPSF) ; NSFC ; CPSF
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66010
专题北京大学药学院
北京大学医学部管理机构_医学部
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
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GB/T 7714
Wu, Li,Wang, Yuan,Wu, Junzhou,et al. Caged circular antisense oligonucleotides for photomodulation of RNA digestion and gene expression in cells[J]. NUCLEIC ACIDS RESEARCH,2013,41(1):677-686.
APA Wu, Li,Wang, Yuan,Wu, Junzhou,Lv, Cong,Wang, Jie,&Tang, Xinjing.(2013).Caged circular antisense oligonucleotides for photomodulation of RNA digestion and gene expression in cells.NUCLEIC ACIDS RESEARCH,41(1),677-686.
MLA Wu, Li,et al."Caged circular antisense oligonucleotides for photomodulation of RNA digestion and gene expression in cells".NUCLEIC ACIDS RESEARCH 41.1(2013):677-686.
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