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学科主题: 基础医学
题名:
Dihydroarteannuin ameliorates lupus symptom of BXSB mice by inhibiting production of TNF-alpha and blocking the signaling pathway NF-kappa B translocation
作者: Li, Wei-Dong; Dong, Yan-Jun; Tu, You-You; Lin, Zhi-Bin
关键词: dihydroarteannuin (DHA) ; TNF-alpha ; NF-kappa B ; I kappa B-alpha ; lupus ; BXSB mice
刊名: INTERNATIONAL IMMUNOPHARMACOLOGY
发表日期: 2006-08-01
DOI: 10.1016/j.intimp.2006.03.004
卷: 6, 期:8, 页:1243-1250
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Pharmacology & Pharmacy
研究领域[WOS]: Immunology ; Pharmacology & Pharmacy
关键词[WOS]: TRANSCRIPTION FACTOR ; AP-1 ACTIVATION ; RECEPTOR ; CELLS
英文摘要:

The aim of this study was to investigate the mechanisms of action of Dihydroarteannuin (DHA), a semi-synthesized agent from the starting material artemisinin extracted from the Chinese Traditional Herbs Artemisia annua, on ameliorating the symptoms of lupus on BXSB mice. The concentration of TNF-alpha in the culture supernatant of the peritoneal macrophages and in the sera of BXSB mice was determined by the ELISA method. NF-kappa B protein expression and translocation were assayed by the EMSA method and laser confocal scanning microscopy method, respectively. I kappa B-alpha and NF-kappa B p65 protein expression were determined by the Western blot method. Renal tissue of the BXSB mice was prepared for assaying inhibitory activity of DHA on NF-kappa B, p65 and I kappa B-alpha protein expression in vivo. The peritoneal macrophages were prepared for analysis inhibitory effects of DHA on translocation of NF-kappa B into nuclear in vitro. We found that DHA strongly reduced the production of TNF-alpha in the culture supernatant of the peritoneal macrophages and in the sera of BXSB mice in vitro or in vivo. The results demonstrated that DHA decreased the expression of NF-kappa B subunit p65 protein and the activation of NF-kappa B in the renal tissue of BXSB mice in vivo. DHA effectively inhibited the nuclear translocation of NF-kappa B in peritoneal macrophages of BXSB mice in vitro. Furthermore, it was demonstrated that the degradation of I kappa B-alpha protein was significantly inhibited by DHA. These observations suggested that the inhibitory effects of DRA on TNF-alpha production may result from the block in the NF-kappa B signaling pathway upstream of I kappa B degradation. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000238874100004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66031
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Beijing 100083, Peoples R China
2.Chinese Acad Tradit Chinese Med, Inst Chinese Mat Med, Beijing 100083, Peoples R China

Recommended Citation:
Li, Wei-Dong,Dong, Yan-Jun,Tu, You-You,et al. Dihydroarteannuin ameliorates lupus symptom of BXSB mice by inhibiting production of TNF-alpha and blocking the signaling pathway NF-kappa B translocation[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2006,6(8):1243-1250.
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