IR@PKUHSC  > 北京大学基础医学院  > 医学信息学系
学科主题医学信息学
Proteomic analysis of metabolic, cytoskeletal and stress response proteins in human heart failure
Li, Weiming1; Rong, Rong2; Zhao, Sheng3; Zhu, Xiaoming1; Zhang, Ke4; Xiong, Xin5; Yu, Xueqing2; Cui, Qinghua6; Li, Shuqiang7,8; Chen, Li9; Cai, Jun1; Du, Jie10
关键词heart failure proteomics metabolism cytoskeleton heat shock protein
刊名JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
2012
DOI10.1111/j.1582-4934.2011.01336.x
16期:1页:59-71
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Medicine, Research & Experimental
研究领域[WOS]Cell Biology ; Research & Experimental Medicine
关键词[WOS]ALPHA-B-CRYSTALLIN ; SPONTANEOUSLY HYPERTENSIVE-RATS ; FAILING HUMAN HEARTS ; NF-KAPPA-B ; DILATED CARDIOMYOPATHY ; LIGHT-CHAIN ; HYPERTROPHIC CARDIOMYOPATHY ; ISCHEMIA/REPERFUSION INJURY ; SHOCK PROTEINS ; BCL-2 HOMOLOG
英文摘要

Human heart failure is a complex syndrome and a primary cause of morbidity and mortality in the world. However, the molecular pathways involved in the remodelling process are poorly understood. In this study, we performed exhaustive global proteomic surveys of cardiac ventricle isolated from failing and non-failing human hearts, and determined the regulatory pathway to uncover the mechanism underlying heart failure. Two-dimensional gel electrophoresis (2-DE) coupled with tandem mass spectrometry was used to identify differentially expressed proteins in specimens from failing (n = 9) and non-failing (n = 6) human hearts. A total of 25 proteins with at least 1.5-fold change in the failing heart were identified; 15 proteins were up-regulated and 10 proteins were down-regulated. The altered proteins belong to three broad functional categories: (i) metabolic [e.g. NADH dehydrogenase (ubiquinone), dihydrolipoamide dehydrogenase, and the cytochrome c oxidase subunit]; (ii) cytoskeletal (e.g. myosin light chain proteins, troponin I type 3 and transthyretin) and (iii) stress response (e.g. aB-crystallin, HSP27 and HSP20). The marked differences in the expression of selected proteins, including HSP27 and HSP20, were further confirmed by Western blot. Thus, we carried out full-scale screening of the protein changes in human heart failure and profiled proteins that may be critical in cardiac dysfunction for future mapping.

语种英语
WOS记录号WOS:000298586600006
项目编号81050012 ; 30888004 ; 30900590 ; 2009B39 ; 7102057
资助机构National Science Foundation of China ; Beijing Nova Program ; Beijing Natural Science Foundation ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, Education Ministry of China
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66038
专题北京大学基础医学院_医学信息学系
北京大学基础医学院
作者单位1.Capital Med Univ, Chaoyang Hosp, Dept Cardiol, Minist Educ,Key Lab Remodelling Related Cardiovas, Beijing 100020, Peoples R China
2.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Nephrol, Guangzhou 510275, Guangdong, Peoples R China
3.Nanjing Med Univ, Affiliated Hosp 1, Dept Cardiac Surg, Nanjing, Peoples R China
4.Tech Univ Munich, German Res Ctr Environm Hlth, Munich, Germany
5.Chongqing Med Univ, Affiliated Hosp 1, Chongqing, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Dept Biomed Informat, Beijing 100871, Peoples R China
7.Harvard Univ, Sch Med, AIDS, Dana Farber Canc Inst, Boston, MA 02115 USA
8.Capital Med Univ, Beijing Anzhen Hosp, Key Lab Remodelling Related Cardiovasc Dis, Minist Educ, Beijing, Peoples R China
9.Harvard Univ, Sch Med, Dept Canc Immunol, Boston, MA 02115 USA
10.Texas Heart Inst, Houston, TX 77025 USA
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GB/T 7714
Li, Weiming,Rong, Rong,Zhao, Sheng,et al. Proteomic analysis of metabolic, cytoskeletal and stress response proteins in human heart failure[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2012,16(1):59-71.
APA Li, Weiming.,Rong, Rong.,Zhao, Sheng.,Zhu, Xiaoming.,Zhang, Ke.,...&Du, Jie.(2012).Proteomic analysis of metabolic, cytoskeletal and stress response proteins in human heart failure.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,16(1),59-71.
MLA Li, Weiming,et al."Proteomic analysis of metabolic, cytoskeletal and stress response proteins in human heart failure".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 16.1(2012):59-71.
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