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学科主题: 医学信息学
题名:
Proteomic analysis of metabolic, cytoskeletal and stress response proteins in human heart failure
作者: Li, Weiming1; Rong, Rong2; Zhao, Sheng3; Zhu, Xiaoming1; Zhang, Ke4; Xiong, Xin5; Yu, Xueqing2; Cui, Qinghua6; Li, Shuqiang7,8; Chen, Li9; Cai, Jun1; Du, Jie10
关键词: heart failure ; proteomics ; metabolism ; cytoskeleton ; heat shock protein
刊名: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
发表日期: 2012
DOI: 10.1111/j.1582-4934.2011.01336.x
卷: 16, 期:1, 页:59-71
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Medicine, Research & Experimental
研究领域[WOS]: Cell Biology ; Research & Experimental Medicine
关键词[WOS]: ALPHA-B-CRYSTALLIN ; SPONTANEOUSLY HYPERTENSIVE-RATS ; FAILING HUMAN HEARTS ; NF-KAPPA-B ; DILATED CARDIOMYOPATHY ; LIGHT-CHAIN ; HYPERTROPHIC CARDIOMYOPATHY ; ISCHEMIA/REPERFUSION INJURY ; SHOCK PROTEINS ; BCL-2 HOMOLOG
英文摘要:

Human heart failure is a complex syndrome and a primary cause of morbidity and mortality in the world. However, the molecular pathways involved in the remodelling process are poorly understood. In this study, we performed exhaustive global proteomic surveys of cardiac ventricle isolated from failing and non-failing human hearts, and determined the regulatory pathway to uncover the mechanism underlying heart failure. Two-dimensional gel electrophoresis (2-DE) coupled with tandem mass spectrometry was used to identify differentially expressed proteins in specimens from failing (n = 9) and non-failing (n = 6) human hearts. A total of 25 proteins with at least 1.5-fold change in the failing heart were identified; 15 proteins were up-regulated and 10 proteins were down-regulated. The altered proteins belong to three broad functional categories: (i) metabolic [e.g. NADH dehydrogenase (ubiquinone), dihydrolipoamide dehydrogenase, and the cytochrome c oxidase subunit]; (ii) cytoskeletal (e.g. myosin light chain proteins, troponin I type 3 and transthyretin) and (iii) stress response (e.g. aB-crystallin, HSP27 and HSP20). The marked differences in the expression of selected proteins, including HSP27 and HSP20, were further confirmed by Western blot. Thus, we carried out full-scale screening of the protein changes in human heart failure and profiled proteins that may be critical in cardiac dysfunction for future mapping.

语种: 英语
所属项目编号: 81050012 ; 30888004 ; 30900590 ; 2009B39 ; 7102057
项目资助者: National Science Foundation of China ; Beijing Nova Program ; Beijing Natural Science Foundation ; Scientific Research Foundation for the Returned Overseas Chinese Scholars, Education Ministry of China
WOS记录号: WOS:000298586600006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66038
Appears in Collections:基础医学院_医学信息学系_期刊论文

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作者单位: 1.Capital Med Univ, Chaoyang Hosp, Dept Cardiol, Minist Educ,Key Lab Remodelling Related Cardiovas, Beijing 100020, Peoples R China
2.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Nephrol, Guangzhou 510275, Guangdong, Peoples R China
3.Nanjing Med Univ, Affiliated Hosp 1, Dept Cardiac Surg, Nanjing, Peoples R China
4.Tech Univ Munich, German Res Ctr Environm Hlth, Munich, Germany
5.Chongqing Med Univ, Affiliated Hosp 1, Chongqing, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Dept Biomed Informat, Beijing 100871, Peoples R China
7.Harvard Univ, Sch Med, AIDS, Dana Farber Canc Inst, Boston, MA 02115 USA
8.Capital Med Univ, Beijing Anzhen Hosp, Key Lab Remodelling Related Cardiovasc Dis, Minist Educ, Beijing, Peoples R China
9.Harvard Univ, Sch Med, Dept Canc Immunol, Boston, MA 02115 USA
10.Texas Heart Inst, Houston, TX 77025 USA

Recommended Citation:
Li, Weiming,Rong, Rong,Zhao, Sheng,et al. Proteomic analysis of metabolic, cytoskeletal and stress response proteins in human heart failure[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2012,16(1):59-71.
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