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学科主题基础医学
Investigate the role of PTEN in chemotaxis of human breast cancer cells
Wan, Wuzhou; Zou, Haixia; Sun, Ronghua; Liu, Ying; Wang, Jingna; Ma, Dalong; Zhang, Ning
关键词PTEN EGFR breast cancer chemotaxis adhesion metastasis
刊名CELLULAR SIGNALLING
2007-11-01
DOI10.1016/j.cellsig.2007.06.007
19期:11页:2227-2236
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]KINASE-C-ZETA ; PROSTATE-CANCER ; METASTASIS ; MECHANISMS ; EXPRESSION ; POLARITY ; BRAIN ; MOUSE ; LEADS
英文摘要

Chemotaxis plays an important role in metastasis of cancer cells. In the current study, we investigated the role of PTEN, a tumor suppressor, in chemotaxis of human breast cancer cells. Over-expression of PTEN inhibited EGF-induced chemotaxis, probably due to an overall reduction of PIP3 levels. Disruption of PTEN by siRNA caused a marked decrease in chemokinesis, cell adhesion, and membrane spreading, resulting in a severe defect in chemotaxis. In PTEN disrupted cells, PDK1, AKT, and PKC zeta exhibited elevated basal activities, which prevented EGF-induced further activation of these molecules. In the absence of EGF, active PDK1 was detected on multiple directions of the plasma membranes of PTEN disrupted cells, which competed against EGF-induced gradient sensing. To confirm the biological relevance of in vitro studies, both PTEN disrupted cells and its parental human breast cancer cells were injected into tail veins of SCID mice. Mice injected with PTEN disrupted cancer cells showed a marked decrease in lung metastasis. Taken together, our data show that PTEN plays a non-redundant role in EGF-induced chemotaxis of human breast cancer cells, and an optimal level of PTEN is required in these responses. (C) 2007 Published by Elsevier Inc.

语种英语
WOS记录号WOS:000250026300003
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66041
专题北京大学基础医学院_免疫学系
作者单位1.Oakland Univ, Dept Biol Sci, Rochester, MI 48063 USA
2.Peking Univ, Coll Chem, Dept Biol Chem, Beijing Natl Lab Mol Sci,State Key Lab Mol Dynam, Beijing 100871, Peoples R China
3.Peking Univ, Coll Chem, Tianjin 100871, Peoples R China
4.Tianjin Med Univ, Canc Inst Hosp, Tianjin 300060, Peoples R China
5.Oakland Univ, Dept Biol Sci, Rochester, MI 48309 USA
6.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing, Peoples R China
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Wan, Wuzhou,Zou, Haixia,Sun, Ronghua,et al. Investigate the role of PTEN in chemotaxis of human breast cancer cells[J]. CELLULAR SIGNALLING,2007,19(11):2227-2236.
APA Wan, Wuzhou.,Zou, Haixia.,Sun, Ronghua.,Liu, Ying.,Wang, Jingna.,...&Zhang, Ning.(2007).Investigate the role of PTEN in chemotaxis of human breast cancer cells.CELLULAR SIGNALLING,19(11),2227-2236.
MLA Wan, Wuzhou,et al."Investigate the role of PTEN in chemotaxis of human breast cancer cells".CELLULAR SIGNALLING 19.11(2007):2227-2236.
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