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Frequent genetic alterations and reduced expression of the Axin1 gene in oral squamous cell carcinoma: Involvement in tumor progression and metastasis
Zhou, Chuan-Xiang; Gao, Yan
关键词Axin1 mutation polymorphism denaturing high performance liquid chromatography immunohistochemistry differentiation metastasis oral squamous cell carcinoma
刊名ONCOLOGY REPORTS
2007
17期:1页:73-79
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]WNT SIGNALING PATHWAY ; ACTIVATED PROTEIN-KINASE ; BETA-CATENIN ; HEPATOCELLULAR CARCINOMAS ; NEGATIVE REGULATOR ; COLORECTAL-CANCER ; MUTATIONS ; DOMAINS ; APC ; PHOSPHORYLATION
英文摘要

The aims of this study were to analyze the genetic alterations and expression levels of the Axin1 gene in oral squamous cell carcinoma (OSCC), to evaluate its clinical importance and to clarify whether the Axin1 gene is involved in the pathogenesis of OSCC. Mutation analysis of the Axinl gene was performed by denaturing high performance liquid chromatography (DHPLC) and DNA sequencing in 44 OSCC samples. Meanwhile, Axinl protein expression was investigated by immunohistochemistry in these samples. Aberrant profiles were detected by DHPLC screening in 26 different OSCC cases. After sequencing analysis, four mutations and five polymorphisms were identified. One case of poorly differentiated OSCC with metastasis contained two mutations: one revealed a T > G substitution at nucleotide 324 in exon 1, resulting in a glycine to stop codon substitution at amino acid residue 108; the other revealed an A > G heterozygous mutation in intron 7, located very near to exon 8. In another two patients with moderately differentiated OSCC and metastasis, a G > T heterozygous mutation at codon 488 in exon 5 and a C > G substitution at the intron 5+26 position was detected, respectively. Five polymorphisms were all frequent and localized at positions of codon 254 (GAT -> GAC), codon 429 (GTC -> ATC), codon 525 (GAC -> GAT), codon 609 (GCT -> GCC), and intron 4+17 (G > A), with a frequency of 39%, 8%, 6%, 13% and 9%, respectively. Immunoreactivity for Axinl was strongly positive in normal stratified squamous epithelium but significantly reduced expression of Axinl was shown in most of the 44 tumor specimens (35/44), especially in poorly differentiated tumors with metastasis. These results suggest that mutational inactivation and reduced expression of the Axinl gene may play a pivotal role in OSCC carcinogenesis and metastasis.

语种英语
WOS记录号WOS:000243114100011
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66044
专题北京大学口腔医学院_口腔病理科
作者单位Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing, Peoples R China
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Zhou, Chuan-Xiang,Gao, Yan. Frequent genetic alterations and reduced expression of the Axin1 gene in oral squamous cell carcinoma: Involvement in tumor progression and metastasis[J]. ONCOLOGY REPORTS,2007,17(1):73-79.
APA Zhou, Chuan-Xiang,&Gao, Yan.(2007).Frequent genetic alterations and reduced expression of the Axin1 gene in oral squamous cell carcinoma: Involvement in tumor progression and metastasis.ONCOLOGY REPORTS,17(1),73-79.
MLA Zhou, Chuan-Xiang,et al."Frequent genetic alterations and reduced expression of the Axin1 gene in oral squamous cell carcinoma: Involvement in tumor progression and metastasis".ONCOLOGY REPORTS 17.1(2007):73-79.
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