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学科主题: 公共卫生
题名:
Induction of cell-cycle arrest by all-trans retinoic acid in mouse embryonic palatal mesenchymal (MEPM) cells
作者: Yu, ZL; Lin, JX; Xiao, Y; Han, J; Zhang, XZ; Jia, HC; Tang, YN; Li, Y
关键词: all-trans retinoic acid ; mouse embryonic palatal mesenchymal cells ; cell cycle ; cyclins ; retinoblastoma protein
刊名: TOXICOLOGICAL SCIENCES
发表日期: 2005-02-01
DOI: 10.1093/toxsci/kfi030
卷: 83, 期:2, 页:349-354
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: RETINOBLASTOMA PROTEIN ; DEPENDENT KINASES ; INDUCED APOPTOSIS ; LEUKEMIA-CELLS ; GROWTH ; PHOSPHORYLATION ; PROLIFERATION ; PATHWAY ; CARCINOMA ; HEDGEHOG
英文摘要:

all-trans retinoic acid (atRA), the oxidative metabolite of vitamin A, is essential for normal embryonic development. Also, high levels of atRA are teratogenic in many species and can effectively induce cleft palate in the mouse. Most cleft palate resulted from the failed fusion of secondary palate shelves, and maintenance of the normal cell proliferation is important in this process of shelf growth. To clarify the mechanism by which atRA causes cleft palate. we investigated the effect of atRA on proliferation activity and cell cycle distribution in mouse embryonic palatal mesenchymal (MEPM) cells. atRA inhibited the growth of MEPM cells by inducing apoptosis in a dose-dependent manner. atRA also caused a G1 block in the cell cycle with an increase in the proportion of cells in G0/G1 and a decrease in the proportion of cells in S phase, as determined by flow cytometry. We next investigated the effects of atRA on molecules that regulate the G1 to S phase transition. These studies demonstrated that atRA inhibited expression of cyclins D and E at the protein level. Further-more, atRA treatment reduced phosphorylated Rb and decreased cdk2 and cdk4 kinase activity. These data suggest that atRA had antiproliferative activity by modulating GIN cell cycle regulators and by inhibition of Rb phosphorylation in MEPM cells, which might account for the pathogenesis of cleft palate induced by retinoic acid.

语种: 英语
WOS记录号: WOS:000226371400016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66050
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Peking Univ, Sch Publ Hlth, Beijing 100083, Peoples R China
2.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Yu, ZL,Lin, JX,Xiao, Y,et al. Induction of cell-cycle arrest by all-trans retinoic acid in mouse embryonic palatal mesenchymal (MEPM) cells[J]. TOXICOLOGICAL SCIENCES,2005,83(2):349-354.
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