|Splenectomy ameliorates acute multiple organ damage induced by liver warm ischemia reperfusion in rats|
|Jiang, Hongchi; Meng, Fanqiang; Li, Wei; Tong, Liquan; Qiao, Haiquan; Sun, Xueying|
|WOS标题词||Science & Technology|
|关键词[WOS]||TUMOR-NECROSIS-FACTOR ; HEPATIC ISCHEMIA/REPERFUSION INJURY ; LUNG INJURY ; PARTIAL-HEPATECTOMY ; FACTOR-ALPHA ; NEUTROPHIL ; INFLAMMATION ; APOPTOSIS ; INTERLEUKIN-1 ; TOLERANCE|
Background. Liver ischemia/reperfusion (I/R) results in the release of destructive proinflammatory cytokines and oxygen-derived radicals, which in turn cause injury to liver and other organs such as kidney, lung, and intestine. Splenectomy protects organs from intestinal I/R injury. Therefore, the present study aims to investigate whether splenectomy could also ameliorate multiple organ damage caused by liver I/R
Methods. Wistar rats randomly assigned into 4 groups underwent sham-operation, splenectomy, hepatic I/R induced by occlusion of hepatic artery and portal vein, and splenectomy plus hepatic I/R respectively. Blood samples were collected for assessing aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity, and tumor necrosis factor-alpha (TNF-alpha) levels. The activity of myeloperoxidase (MPO) in liver tissues was assessed. Livers, kidneys, lungs, and small intestines underwent histopathologic examination for scoring injury severity and TUNEL assay for cell apoptosis. The expression of caspase-3 was evaluated with Western blot analysis.
Results. Liver I/R resulted in liver injury as evidenced by morphologic abnormalities, increased serum activities of AST and ALT, and increased Percentage of apoptotic cells. The activity of MPO in liver tissues and the serum Levels of TAT-a were increased after I/R Splenectomy significantly decreased the histologic severity score, apoptotic index, MPO activism and serum Levels of AST, ALT, and TAT-a. Hepatic I/R also caused damage to kidneys, lungs, and small intestines, as evaluated by histologic alterations and increased apoptotic cells; these changes were ameliorated by splenectomy. The expression of caspase-3 was upregulated in the 4 organs by hepatic I/R and inhibited by splenectomy.
Conclusions. Splenectomy protects the liver as well as the kidney, lung, and intestine from injury by hepatic I/R. Although the mechanism needs further investigation, this study demonstrated that splenectomy inhibited leukocyte infiltration in livers, release of TAT-a, cell apoptosis, and expression of caspase-3.
|作者单位||1.Harbin Med Coll, Clin Coll 1, Hepatosplen Surg Ctr, Dept Gen Surg, Harbin 150001, Peoples R China|
2.Peking Univ, Gynecol Canc Ctr, Peoples Hosp, Beijing 100871, Peoples R China
|Jiang, Hongchi,Meng, Fanqiang,Li, Wei,et al. Splenectomy ameliorates acute multiple organ damage induced by liver warm ischemia reperfusion in rats[J]. SURGERY,2007,141(1):32-40.|
|APA||Jiang, Hongchi,Meng, Fanqiang,Li, Wei,Tong, Liquan,Qiao, Haiquan,&Sun, Xueying.(2007).Splenectomy ameliorates acute multiple organ damage induced by liver warm ischemia reperfusion in rats.SURGERY,141(1),32-40.|
|MLA||Jiang, Hongchi,et al."Splenectomy ameliorates acute multiple organ damage induced by liver warm ischemia reperfusion in rats".SURGERY 141.1(2007):32-40.|