Institutional Repository of Peking University School of Basic Medical Sciences
|Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery|
|Han, Jing-Yan; Horie, Yoshinori; Miura, Soichiro; Akiba, Yasutada; Guo, Jun; Li, Dan; Fan, Jing-Yu; Liu, Yu-Ying; Hu, Bai-He; An, Li-Hua; Chang, Xin; Xu, Man; Guo, De-An; Sun, Kai; Yang, Ji-Ying; Fang, Shu-Ping; Xian, Ming-Ji; Kizaki, Masahiro; Nagata, Hiroshi; Hibi, Toshifumi|
|关键词||compound danshen mesenteric microcirculation oxygen-free radical adhesion molecules lipopolysaccharide|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||NECROSIS-FACTOR-ALPHA ; INTERCELLULAR-ADHESION MOLECULE-1 ; NF-KAPPA-B ; ENDOTHELIAL CELL-INTERACTIONS ; SALVIANOLIC-ACID-B ; MURINE MAST-CELLS ; I DOMAIN ; P-SELECTIN ; MICROVASCULAR DYSFUNCTION ; NEUTROPHIL ADHERENCE|
AIM: To investigate the effect of compound Danshen injection on lipopolysaccharide (LPS)-induced rat mesenteric microcirculatory dysfunctions and the underlying possible mechanism by an inverted intravital microscope and high-speed video camera system.
METHODS: LPS was continuously infused through the jugular artery of male Wistar rats at the dose of 2 mg/kg per hour. Changes in mesenteric microcirculation, such as diameters of arterioles and venules, velocity of RBCs in venules, leukocyte rolling, adhesion and emigration, free radicals released from post-capillary venules, FITC-albumin leakage and mast cell degranulation, were observed through an inverted intravital microscope assisted with CCD camera and SIT camera. Meanwhile, the expression of adhesion molecules CD11b/CD18 and the production of free radical in neutrophils, and the expression of intercellular adhesion molecule 1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) were quantified by flow cytometry (FACS) in vitro.
RESULTS: The continuous infusion with LPS resulted in a number of responses in microcirculation, including a significant increase in the positive region of venule stained with Monastral blue 13, rolling and adhesion of leukocytes, production of oxygen radical in venular wall, albumin efflux and enhanced mast cell degranulation in vivo, all of which, except for the leukocyte rolling, were attenuated by the treatment with compound Danshen injection. Experiments performed in vitro further revealed that the expression of CD11b/CD18 and the production of oxygen free radical in neutrophils, and the expression of ICAM-1 in HUVECs were increased by exposure to LPS, and they were attenuated by compound Danshen injection.
CONCLUSION: These results suggest that compound Danshen injection is an efficient drug with multi-targeting potential for improving the microcirculatory disturbance. (c) 2007 WJG. All rights reserved.
|作者单位||1.Keio Univ, Sch Med, Dept Internal Med, Tokyo 1608582, Japan|
2.Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100083, Peoples R China
3.Natl Def Med Coll, Dept Internal Med 2, Saitama 3598513, Japan
4.Peking Univ, Hlth Sci Ctr, Tasly Microcirculat Res Ctr, Beijing 100083, Peoples R China
|Han, Jing-Yan,Horie, Yoshinori,Miura, Soichiro,et al. Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2007,13(26):3581-3591.|
|APA||Han, Jing-Yan.,Horie, Yoshinori.,Miura, Soichiro.,Akiba, Yasutada.,Guo, Jun.,...&Hibi, Toshifumi.(2007).Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery.WORLD JOURNAL OF GASTROENTEROLOGY,13(26),3581-3591.|
|MLA||Han, Jing-Yan,et al."Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery".WORLD JOURNAL OF GASTROENTEROLOGY 13.26(2007):3581-3591.|