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学科主题: 基础医学
题名:
Gefitinib-sensitizing mutation in esophageal carcinoma cell line Kyse450
作者: Guo, MZ; Liu, S; Herman, JG; Zhuang, H; Lu, FM
关键词: gefitinib ; esophageal cancer ; EGFR ; mutations
刊名: CANCER BIOLOGY & THERAPY
发表日期: 2006-02-01
卷: 5, 期:2, 页:152-155
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: GROWTH-FACTOR RECEPTOR ; TYROSINE KINASE INHIBITOR ; LUNG-CANCER ; CHINESE PATIENTS ; EGFR MUTATIONS ; IRESSA ; ZD1839 ; RESPONSIVENESS ; PREVENTION ; P27(KIP1)
英文摘要:

Purpose: The sensitivity of lung cancer to gefitinib has been found to be associated with mutations at the tyrosine kinase domain of epidermal growth factor receptor (EGFR), yet similar observations are not available in other solid tumors. We recently identified mutations in the EGFR kinase domain in primary esophageal carcinoma. The purpose of this study was to investigate if they are gefitinib-sensitizing EGFR mutations.

Experimental design: We identified a missense mutation in the EGFR kinase domain, EGRFS7681, in the esophageal cancer cell line Kyse450. The senstivity of this cell line to gefitinib was compared to an esophageal cancer cell line with wildtype EGFR, TE8, and to a lung cancer cell line, H358, known to be resistent to gefitinib. The effect of EGFR(S768I) mutation on cell growth and apoptosis was assessed.

Results: As demonstrated by in vitro proliferation assay, this mutation sensitized Kyse450 cells to gefitinib. The observation of down regulation of the phosphorylated pAKT indicated gefitinib induced Kyse450 cells apoptosis via inhibition of EGFR activity

Conclusions: While more primary esophageal tumors remain to be screened for the mutations at the tyrosine kinase domain of EGFR, current observation implies that gefitinib may be worth further investigation for treatment of esophageal cancers.

语种: 英语
WOS记录号: WOS:000236044600013
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66063
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Johns Hopkins, Sidney Kimmel Canc Ctr, Baltimore, MD USA
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China
3.Zhengzhou Univ, Teaching Hosp 1, Zhengzhou 450052, Peoples R China

Recommended Citation:
Guo, MZ,Liu, S,Herman, JG,et al. Gefitinib-sensitizing mutation in esophageal carcinoma cell line Kyse450[J]. CANCER BIOLOGY & THERAPY,2006,5(2):152-155.
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