学科主题临床医学
Determination of the Human Antibody Response to the Neutralization Epitopes Encompassing Amino Acids 313-327 and 432-443 of Hepatitis C Virus E1E2 Glycoproteins
Liu, Ruyu1,2; Rao, Huiying1,2; Wang, Jianghua1,2; Xie, Xingwang1,2; Jiang, Dong1,2; Pan, Xiaoben1,2; Zhao, Ping3; Zhang, Henghui1,2; Wei, Lai1,2
刊名PLOS ONE
2013-06-24
DOI10.1371/journal.pone.0066872
8期:6
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MONOCLONAL-ANTIBODY ; VIRAL CLEARANCE ; SINGLE-SOURCE ; CELL ENTRY ; INFECTION ; PARTICLES ; HCV ; E2 ; ASSOCIATION ; INDUCTION
英文摘要

It has been reported that monoclonal antibodies (MAbs) to the E1E2 glycoproteins may have the potential to prevent hepatitis C virus (HCV) infection. The protective epitopes targeted by these MAbs have been mapped to the region sencompassing amino acids 313-327 and 432-443. In this study, we synthesized these two peptides and tested the reactivity of serum samples from 336 patients, 210 of which were from Chronic Hepatitis C (CHC) patients infected with diverse HCV genotypes. The remaining 126 samples were isolated from patients who had spontaneously cleared HCV infection. In the chronic HCV-infected group (CHC group), the prevalence of human serum antibodies reactive to epitopes 313-327 and 432-443 was 24.29% (51 of 210) and 4.76% (10 of 210), respectively. In the spontaneous clearance group (SC group), the prevalence was 0.79% (1 of 126) and 12.70% (16 of 126), respectively. The positive serum samples that contained antibodies reactive to epitope 313-327 neutralized HCV pseudo particles (HCVpp) bearing the envelope glycoproteins of genotypes 1a or 1b and/or 4, but genotypes 2a, 3a, 5 and 6 were not neutralized. The neutralizing activity of these serum samples could not be inhibited by peptide 313-327. Six samples (SC17, SC38, SC86, SC92, CHC75 and CHC198) containing antibodies reactive to epitope 432-443 had cross-genotype neutralizing activities. The neutralizing activity of SC38, SC86, SC92 and CHC75 was partially inhibited by peptide 432-443. However, the neutralizing activity of sample SC17 for genotype 4HCVpp and sample CHC198 for genotype 1b HCVppwere notinhibited by the peptide. This study identifies the neutralizing ability of endogenous anti-HCV antibodies and warrants the exploration of antibodies reactive to epitope 432-443 as sources for future antibody therapies.

语种英语
WOS记录号WOS:000321738400074
项目编号2012ZX10002003
资助机构National Science and Technology Major Project
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66076
专题北京大学第二临床医学院_北京大学肝病研究所
北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100871, Peoples R China
2.Beijing Key Lab Hepatitis C & Immunotherapy Liver, Beijing, Peoples R China
3.Second Mil Med Univ, Shanghai Key Lab Med Biodef, Dept Microbiol, Shanghai, Peoples R China
推荐引用方式
GB/T 7714
Liu, Ruyu,Rao, Huiying,Wang, Jianghua,et al. Determination of the Human Antibody Response to the Neutralization Epitopes Encompassing Amino Acids 313-327 and 432-443 of Hepatitis C Virus E1E2 Glycoproteins[J]. PLOS ONE,2013,8(6).
APA Liu, Ruyu.,Rao, Huiying.,Wang, Jianghua.,Xie, Xingwang.,Jiang, Dong.,...&Wei, Lai.(2013).Determination of the Human Antibody Response to the Neutralization Epitopes Encompassing Amino Acids 313-327 and 432-443 of Hepatitis C Virus E1E2 Glycoproteins.PLOS ONE,8(6).
MLA Liu, Ruyu,et al."Determination of the Human Antibody Response to the Neutralization Epitopes Encompassing Amino Acids 313-327 and 432-443 of Hepatitis C Virus E1E2 Glycoproteins".PLOS ONE 8.6(2013).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Ruyu]的文章
[Rao, Huiying]的文章
[Wang, Jianghua]的文章
百度学术
百度学术中相似的文章
[Liu, Ruyu]的文章
[Rao, Huiying]的文章
[Wang, Jianghua]的文章
必应学术
必应学术中相似的文章
[Liu, Ruyu]的文章
[Rao, Huiying]的文章
[Wang, Jianghua]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。