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学科主题: 临床医学
题名:
Determination of the Human Antibody Response to the Neutralization Epitopes Encompassing Amino Acids 313-327 and 432-443 of Hepatitis C Virus E1E2 Glycoproteins
作者: Liu, Ruyu1,2; Rao, Huiying1,2; Wang, Jianghua1,2; Xie, Xingwang1,2; Jiang, Dong1,2; Pan, Xiaoben1,2; Zhao, Ping3; Zhang, Henghui1,2; Wei, Lai1,2
刊名: PLOS ONE
发表日期: 2013-06-24
DOI: 10.1371/journal.pone.0066872
卷: 8, 期:6
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: MONOCLONAL-ANTIBODY ; VIRAL CLEARANCE ; SINGLE-SOURCE ; CELL ENTRY ; INFECTION ; PARTICLES ; HCV ; E2 ; ASSOCIATION ; INDUCTION
英文摘要:

It has been reported that monoclonal antibodies (MAbs) to the E1E2 glycoproteins may have the potential to prevent hepatitis C virus (HCV) infection. The protective epitopes targeted by these MAbs have been mapped to the region sencompassing amino acids 313-327 and 432-443. In this study, we synthesized these two peptides and tested the reactivity of serum samples from 336 patients, 210 of which were from Chronic Hepatitis C (CHC) patients infected with diverse HCV genotypes. The remaining 126 samples were isolated from patients who had spontaneously cleared HCV infection. In the chronic HCV-infected group (CHC group), the prevalence of human serum antibodies reactive to epitopes 313-327 and 432-443 was 24.29% (51 of 210) and 4.76% (10 of 210), respectively. In the spontaneous clearance group (SC group), the prevalence was 0.79% (1 of 126) and 12.70% (16 of 126), respectively. The positive serum samples that contained antibodies reactive to epitope 313-327 neutralized HCV pseudo particles (HCVpp) bearing the envelope glycoproteins of genotypes 1a or 1b and/or 4, but genotypes 2a, 3a, 5 and 6 were not neutralized. The neutralizing activity of these serum samples could not be inhibited by peptide 313-327. Six samples (SC17, SC38, SC86, SC92, CHC75 and CHC198) containing antibodies reactive to epitope 432-443 had cross-genotype neutralizing activities. The neutralizing activity of SC38, SC86, SC92 and CHC75 was partially inhibited by peptide 432-443. However, the neutralizing activity of sample SC17 for genotype 4HCVpp and sample CHC198 for genotype 1b HCVppwere notinhibited by the peptide. This study identifies the neutralizing ability of endogenous anti-HCV antibodies and warrants the exploration of antibodies reactive to epitope 432-443 as sources for future antibody therapies.

语种: 英语
所属项目编号: 2012ZX10002003
项目资助者: National Science and Technology Major Project
WOS记录号: WOS:000321738400074
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66076
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100871, Peoples R China
2.Beijing Key Lab Hepatitis C & Immunotherapy Liver, Beijing, Peoples R China
3.Second Mil Med Univ, Shanghai Key Lab Med Biodef, Dept Microbiol, Shanghai, Peoples R China

Recommended Citation:
Liu, Ruyu,Rao, Huiying,Wang, Jianghua,et al. Determination of the Human Antibody Response to the Neutralization Epitopes Encompassing Amino Acids 313-327 and 432-443 of Hepatitis C Virus E1E2 Glycoproteins[J]. PLOS ONE,2013,8(6).
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