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学科主题口腔医学
The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor
Wang, H-C; Li, T-J
关键词tumor stroma fibroblast keratocystic odontogenic tumor osteoclastogenesis COX-2 VEGF
刊名ORAL DISEASES
2013-03-01
DOI10.1111/j.1601-0825.2012.01966.x
19期:2页:162-168
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]CELLS ; EXPRESSION ; CANCER ; STROMA ; BONE ; DIFFERENTIATION ; INTERLEUKIN-6 ; ACTIVATION ; BEHAVIOR ; LESIONS
英文摘要

OBJECTIVES: To investigate the growth characteristics and effects on osteoclastogenesis in fibroblasts isolated from keratocystic odontogenic tumor (KCOT) fibrous capsule.

MATERIALS AND METHODS: Fibroblasts isolated from KCOT fibrous capsule and normal gingival mucosa were cultured in vitro. Their colony-forming units and proliferative activity were investigated, and the osteoclastogenic effects were also observed by a co-culture system with osteoclast precursor cell line Raw264.7. The mRNA of several genes related to bone resorption (IL-6, VEGF, COX-2, and M-CSF) was analyzed by real-time PCR.

RESULTS: Keratocystic odontogenic tumor fibroblasts developed fewer CFU and had longer population doubling time than gingival fibroblasts (P < 0.05). In contrast to gingival fibroblasts, KCOT fibroblasts expressed less IL-6, COX-2, and M-CSF (P < 0.05); however, the Raw264.7 co-cultured with KCOT fibroblasts developed more osteoclast-like cells and expressed higher level of nfatc1 than that co-cultured with gingival fibroblasts. Increased COX-2 expression and VEGF expression were detected in KCOT fibroblasts and Raw264.7 co-culture system (P < 0.05).

CONCLUSION: Although KCOT fibroblasts showed lower level of cell proliferation than gingival fibroblasts, higher osteoclastogenic ability was detected when co-cultured with Raw264.7. These results suggest that the cell-cell interaction in the co-culture system, possibly by increasing COX-2 and VEGF expression, may be responsible for the increased osteoclastogenic effects of KCOT fibroblasts. Oral Diseases (2013) 19, 162-168

语种英语
WOS记录号WOS:000317489000006
项目编号81030018 ; 30572048 ; 30872900
资助机构National Nature Science Foundation of China
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66090
专题北京大学口腔医学院_口腔病理科
作者单位Peking Univ, Dept Oral Pathol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
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Wang, H-C,Li, T-J. The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor[J]. ORAL DISEASES,2013,19(2):162-168.
APA Wang, H-C,&Li, T-J.(2013).The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor.ORAL DISEASES,19(2),162-168.
MLA Wang, H-C,et al."The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor".ORAL DISEASES 19.2(2013):162-168.
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