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学科主题: 口腔医学
题名:
The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor
作者: Wang, H-C; Li, T-J
关键词: tumor stroma ; fibroblast ; keratocystic odontogenic tumor ; osteoclastogenesis ; COX-2 ; VEGF
刊名: ORAL DISEASES
发表日期: 2013-03-01
DOI: 10.1111/j.1601-0825.2012.01966.x
卷: 19, 期:2, 页:162-168
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: CELLS ; EXPRESSION ; CANCER ; STROMA ; BONE ; DIFFERENTIATION ; INTERLEUKIN-6 ; ACTIVATION ; BEHAVIOR ; LESIONS
英文摘要:

OBJECTIVES: To investigate the growth characteristics and effects on osteoclastogenesis in fibroblasts isolated from keratocystic odontogenic tumor (KCOT) fibrous capsule.

MATERIALS AND METHODS: Fibroblasts isolated from KCOT fibrous capsule and normal gingival mucosa were cultured in vitro. Their colony-forming units and proliferative activity were investigated, and the osteoclastogenic effects were also observed by a co-culture system with osteoclast precursor cell line Raw264.7. The mRNA of several genes related to bone resorption (IL-6, VEGF, COX-2, and M-CSF) was analyzed by real-time PCR.

RESULTS: Keratocystic odontogenic tumor fibroblasts developed fewer CFU and had longer population doubling time than gingival fibroblasts (P < 0.05). In contrast to gingival fibroblasts, KCOT fibroblasts expressed less IL-6, COX-2, and M-CSF (P < 0.05); however, the Raw264.7 co-cultured with KCOT fibroblasts developed more osteoclast-like cells and expressed higher level of nfatc1 than that co-cultured with gingival fibroblasts. Increased COX-2 expression and VEGF expression were detected in KCOT fibroblasts and Raw264.7 co-culture system (P < 0.05).

CONCLUSION: Although KCOT fibroblasts showed lower level of cell proliferation than gingival fibroblasts, higher osteoclastogenic ability was detected when co-cultured with Raw264.7. These results suggest that the cell-cell interaction in the co-culture system, possibly by increasing COX-2 and VEGF expression, may be responsible for the increased osteoclastogenic effects of KCOT fibroblasts. Oral Diseases (2013) 19, 162-168

语种: 英语
所属项目编号: 81030018 ; 30572048 ; 30872900
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000317489000006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66090
Appears in Collections:北京大学口腔医学院_口腔病理科_期刊论文

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作者单位: Peking Univ, Dept Oral Pathol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China

Recommended Citation:
Wang, H-C,Li, T-J. The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor[J]. ORAL DISEASES,2013,19(2):162-168.
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