|The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor|
|Wang, H-C; Li, T-J|
|关键词||tumor stroma fibroblast keratocystic odontogenic tumor osteoclastogenesis COX-2 VEGF|
|WOS标题词||Science & Technology|
|类目[WOS]||Dentistry, Oral Surgery & Medicine|
|研究领域[WOS]||Dentistry, Oral Surgery & Medicine|
|关键词[WOS]||CELLS ; EXPRESSION ; CANCER ; STROMA ; BONE ; DIFFERENTIATION ; INTERLEUKIN-6 ; ACTIVATION ; BEHAVIOR ; LESIONS|
OBJECTIVES: To investigate the growth characteristics and effects on osteoclastogenesis in fibroblasts isolated from keratocystic odontogenic tumor (KCOT) fibrous capsule.
MATERIALS AND METHODS: Fibroblasts isolated from KCOT fibrous capsule and normal gingival mucosa were cultured in vitro. Their colony-forming units and proliferative activity were investigated, and the osteoclastogenic effects were also observed by a co-culture system with osteoclast precursor cell line Raw264.7. The mRNA of several genes related to bone resorption (IL-6, VEGF, COX-2, and M-CSF) was analyzed by real-time PCR.
RESULTS: Keratocystic odontogenic tumor fibroblasts developed fewer CFU and had longer population doubling time than gingival fibroblasts (P < 0.05). In contrast to gingival fibroblasts, KCOT fibroblasts expressed less IL-6, COX-2, and M-CSF (P < 0.05); however, the Raw264.7 co-cultured with KCOT fibroblasts developed more osteoclast-like cells and expressed higher level of nfatc1 than that co-cultured with gingival fibroblasts. Increased COX-2 expression and VEGF expression were detected in KCOT fibroblasts and Raw264.7 co-culture system (P < 0.05).
CONCLUSION: Although KCOT fibroblasts showed lower level of cell proliferation than gingival fibroblasts, higher osteoclastogenic ability was detected when co-cultured with Raw264.7. These results suggest that the cell-cell interaction in the co-culture system, possibly by increasing COX-2 and VEGF expression, may be responsible for the increased osteoclastogenic effects of KCOT fibroblasts. Oral Diseases (2013) 19, 162-168
|项目编号||81030018 ; 30572048 ; 30872900|
|资助机构||National Nature Science Foundation of China|
|作者单位||Peking Univ, Dept Oral Pathol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China|
|Wang, H-C,Li, T-J. The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor[J]. ORAL DISEASES,2013,19(2):162-168.|
|APA||Wang, H-C,&Li, T-J.(2013).The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor.ORAL DISEASES,19(2),162-168.|
|MLA||Wang, H-C,et al."The growth and osteoclastogenic effects of fibroblasts isolated from keratocystic odontogenic tumor".ORAL DISEASES 19.2(2013):162-168.|