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学科主题: 公共卫生
题名:
In vitro malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene
作者: Zhao, Peng1,2; Fu, Juanling1; Yao, Biyun1,2; Song, Yanchao1,2; Mi, Lan1,2; Li, Zhenning1,2; Shang, Lanqin1,2; Hao, Weidong1,2; Zhou, Zongcan1
关键词: Benzo(a)pyrene ; 16HBE cells ; Malignant transformation ; Cell migration ; p53 ; p-Akt (Ser473)
刊名: TOXICOLOGY IN VITRO
发表日期: 2012-03-01
DOI: 10.1016/j.tiv.2011.12.013
卷: 26, 期:2, 页:362-368
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: POLYCYCLIC AROMATIC-HYDROCARBONS ; TUMOR PROTEIN D52 ; LUNG-CANCER ; PROTEOMIC ANALYSIS ; SIGNALING PATHWAY ; EXPRESSION ; HALLMARKS ; G0/G1
英文摘要:

In this study, the human bronchial epithelial cells (16HBE) were treated five times with 10 mu M benzo(a)pyrene (BaP), followed by 20 passages culture, and the in vitro BaP-induced malignant transformation of 16HBE cells was established. Five colonies in soft agarose were then amplified and donated as T-16HBE-C1 similar to 5 cells, respectively. T-16HBE-C1 similar to 5 cells can form tumors subcutaneously in nude mice. Histopathological changes in the tumors indicated nests growth, high nuclear-cytoplasmic ratios, coarse and clumped chromatin, numerous and distinctly atypical mitoses, cell necrosis and surrounding normal adipose, muscle and connective tissue immersed. In addition, lung metastasis was observed in nude mice in T-16HBE-C1, 3 and 4 groups. In vitro cell migration assay results indicated that T-16HBE-C2 similar to 5 cells showed much lower migration capabilities than 16HBE cells. Western blotting analysis showed that the expressions of p53 and p-Akt (Ser473) in T-16HBE-C1 similar to 5 cells were significant higher than those in 16HBE cells. Our results demonstrated that BaP could induce the malignant transformation of 16HBE cells, and p53 and p-Akt (Ser473) might play crucial roles in BaP-induced carcinogenesis. The five monoclonal cell lines (T-16HBE-C1 similar to 5) with different migration capabilities could be used as research models for further understanding the mechanisms of BaP-induced carcinogenesis and cell migration. (C) 2011 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2009ZX09301-010 ; 2009ZX09301-304 ; 30471470 ; 81001253 ; BMU20090460
项目资助者: National Science and Technology Major Specific Project of the People&prime ; s Republic of China ; National Nature Science Foundation of China ; Fundamental Research Funds for the Central Universities
WOS记录号: WOS:000301019600020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66092
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Toxicol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Beijing Key Lab Toxicol Res & Risk Assessment Foo, Beijing 100191, Peoples R China

Recommended Citation:
Zhao, Peng,Fu, Juanling,Yao, Biyun,et al. In vitro malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene[J]. TOXICOLOGY IN VITRO,2012,26(2):362-368.
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