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In vitro malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene
Zhao, Peng1,2; Fu, Juanling1; Yao, Biyun1,2; Song, Yanchao1,2; Mi, Lan1,2; Li, Zhenning1,2; Shang, Lanqin1,2; Hao, Weidong1,2; Zhou, Zongcan1
关键词Benzo(a)pyrene 16HBE cells Malignant transformation Cell migration p53 p-Akt (Ser473)
刊名TOXICOLOGY IN VITRO
2012-03-01
DOI10.1016/j.tiv.2011.12.013
26期:2页:362-368
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]POLYCYCLIC AROMATIC-HYDROCARBONS ; TUMOR PROTEIN D52 ; LUNG-CANCER ; PROTEOMIC ANALYSIS ; SIGNALING PATHWAY ; EXPRESSION ; HALLMARKS ; G0/G1
英文摘要

In this study, the human bronchial epithelial cells (16HBE) were treated five times with 10 mu M benzo(a)pyrene (BaP), followed by 20 passages culture, and the in vitro BaP-induced malignant transformation of 16HBE cells was established. Five colonies in soft agarose were then amplified and donated as T-16HBE-C1 similar to 5 cells, respectively. T-16HBE-C1 similar to 5 cells can form tumors subcutaneously in nude mice. Histopathological changes in the tumors indicated nests growth, high nuclear-cytoplasmic ratios, coarse and clumped chromatin, numerous and distinctly atypical mitoses, cell necrosis and surrounding normal adipose, muscle and connective tissue immersed. In addition, lung metastasis was observed in nude mice in T-16HBE-C1, 3 and 4 groups. In vitro cell migration assay results indicated that T-16HBE-C2 similar to 5 cells showed much lower migration capabilities than 16HBE cells. Western blotting analysis showed that the expressions of p53 and p-Akt (Ser473) in T-16HBE-C1 similar to 5 cells were significant higher than those in 16HBE cells. Our results demonstrated that BaP could induce the malignant transformation of 16HBE cells, and p53 and p-Akt (Ser473) might play crucial roles in BaP-induced carcinogenesis. The five monoclonal cell lines (T-16HBE-C1 similar to 5) with different migration capabilities could be used as research models for further understanding the mechanisms of BaP-induced carcinogenesis and cell migration. (C) 2011 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000301019600020
项目编号2009ZX09301-010 ; 2009ZX09301-304 ; 30471470 ; 81001253 ; BMU20090460
资助机构National Science and Technology Major Specific Project of the People&prime ; s Republic of China ; National Nature Science Foundation of China ; Fundamental Research Funds for the Central Universities
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66092
专题北京大学公共卫生学院_公共卫生学院
北京大学公共卫生学院_毒理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Toxicol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Beijing Key Lab Toxicol Res & Risk Assessment Foo, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Peng,Fu, Juanling,Yao, Biyun,et al. In vitro malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene[J]. TOXICOLOGY IN VITRO,2012,26(2):362-368.
APA Zhao, Peng.,Fu, Juanling.,Yao, Biyun.,Song, Yanchao.,Mi, Lan.,...&Zhou, Zongcan.(2012).In vitro malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene.TOXICOLOGY IN VITRO,26(2),362-368.
MLA Zhao, Peng,et al."In vitro malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene".TOXICOLOGY IN VITRO 26.2(2012):362-368.
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