|Expression of CD147 and matrix metalloproteinase-11 in colorectal cancer and their relationship to clinicopathological features|
|Tian, Xiuyun1; Ye, Chunxiang1,2; Yang, Yongyong3; Guan, Xiaoya1; Dong, Bin1; Zhao, Min1; Hao, Chunyi1|
|关键词||cancer Immunohistochemistry CD147 MMP-11 Prognosis|
|刊名||JOURNAL OF TRANSLATIONAL MEDICINE|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, Research & Experimental|
|研究领域[WOS]||Research & Experimental Medicine|
|关键词[WOS]||POOR-PROGNOSIS ; HUMAN STROMELYSIN-3 ; BREAST-CANCER ; CARCINOMA ; CELLS ; INDUCER ; OVEREXPRESSION ; ADENOCARCINOMA ; EMMPRIN ; MICROENVIRONMENT|
Background: This study aimed to investigate the expression of CD147 and MMP-11 in human colorectal cancer (CRC) and to evaluate their clinical significance.
Methods: Real-time polymerase chain reaction was used to evaluate CD147 and MMP-11 mRNA level in 56 pairs of fresh CRC samples matched with adjacent normal mucosa. The protein expression of CD147 and MMP-11 in CRC specimens and corresponding normal colorectal mucosa were evaluated by immunohistochemistry on CRC tissue microarrays. Expression and co-localization of these two proteins in human colorectal cancer tissue were also evaluated by laser scanning confocal microscopy. Furthermore, their correlations with clinicopathological factors and overall survival after surgery were evaluated.
Results: Both CD147 and MMP-11 were demonstrated to be over-expressed at mRNA level (P < 0.001, both) and protein level (P < 0.001, both) in CRC tissue than paired normal mucosa. Spearman rank test showed a positive correlation between these two proteins (P = 0.025). Immunofluorescence double staining confirmed the co-localization of CD147 and MMP-11 in paraffin-embedded tissues of CRC patients. Expression of CD147 and MMP-11 were both correlated with CRC lymph node metastasis (P = 0.021 and P = 0.031, respectively), distant metastasis (P < 0.001 and P = 0.013, respectively) and TNM stage (P = 0.006 and P = 0.049, respectively). Univariate survival analysis showed that both CD147 and MMP11 expression was significantly associated with shorter survival time (P = 0.001 and P = 0.009, respectively). Additionally, in multivariate analysis, both CD147 and MMP-11 were proved to be independent prognostic factors (P = 0.009, 0.028, respectively).
Conclusions: These results indicated that both CD147 and MMP-11 may be involved in the progression of colorectal cancer, and they are potential prognostic factors and might become new therapeutic targets for CRC patients.
|项目编号||2013DFG32720 ; 7153161 ; 81441071 ; 61372028 ; 81272765 ; Z121107001012083|
|资助机构||International Science & ; Technology Cooperation Program of China ; Beijing Municipal Natural Science Foundation ; National natural science funding ; Capital characteristic clinical application research|
|作者单位||1.Peking Univ, Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Minist Educ Beijing,Dept Hepatopancreatobiliary S, Beijing 100871, Peoples R China|
2.Peking Univ, Peoples Hosp, Dept Surg Gastroenterol, Beijing 100871, Peoples R China
3.Peking Univ, Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Minist Educ Beijing,Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
|Tian, Xiuyun,Ye, Chunxiang,Yang, Yongyong,et al. Expression of CD147 and matrix metalloproteinase-11 in colorectal cancer and their relationship to clinicopathological features[J]. JOURNAL OF TRANSLATIONAL MEDICINE,2015,13.|
|APA||Tian, Xiuyun.,Ye, Chunxiang.,Yang, Yongyong.,Guan, Xiaoya.,Dong, Bin.,...&Hao, Chunyi.(2015).Expression of CD147 and matrix metalloproteinase-11 in colorectal cancer and their relationship to clinicopathological features.JOURNAL OF TRANSLATIONAL MEDICINE,13.|
|MLA||Tian, Xiuyun,et al."Expression of CD147 and matrix metalloproteinase-11 in colorectal cancer and their relationship to clinicopathological features".JOURNAL OF TRANSLATIONAL MEDICINE 13(2015).|