IR@PKUHSC  > 北京大学第一临床医学院  > 普通外科
学科主题临床医学
Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis
Hao, Kun; Tian, Xiao-Dong; Qin, Chang-Fu; Xie, Xue-Hai; Yang, Yin-Mo
关键词Hedgehog pancreatic cancer cancer stem cell epithelial-mesenchymal transition
刊名ONCOLOGY REPORTS
2013-03-01
DOI10.3892/or.2012.2210
29期:3页:1124-1132
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]STEM-CELLS ; SONIC HEDGEHOG ; TUMOR-GROWTH ; ACTIVATION ; INVASION ; CARCINOGENESIS ; IDENTIFICATION ; INHIBITION ; EXPRESSION ; CARCINOMA
英文摘要

Pancreatic cancer is one of the most aggressive and devastating malignancies. The Hedgehog (Hh) pathway has been reported to play an important role in pancreatic cancer development and progression. The aim of this study was to examine the activation of the Hh pathway in human pancreatic cancer tissue samples and pancreatic cancer cell lines, and the molecular mechanisms involved in the Hh pathway mediated effects on pancreatic cancer cell proliferation and invasion. The expression levels of Hh molecules in human pancreatic cancer tissue samples and pancreatic cancer cell lines were evaluated using RT-PCR. The role of the Hh pathway in cell proliferation and invasion was evaluated using flow cytometry, MTT, colony formation assays and Transwell invasion assays, and the expression of cancer stem cell markers and epithelial-mesenchymal transition (EMT) were evaluated using flow cytometry and RT-PCR. Tumorigenicity assays were used to further investigate the role of the Hh pathway in vivo. Hh molecules were highly expressed in human pancreatic cancer tissue samples and pancreatic cancer cell lines. Inhibition of the Hh pathway notably decreased cell proliferation and induced apoptosis through inhibition of the PI3K/AKT pathway and cancer stem cells. Furthermore, inhibition of the Hh signaling pathway significantly inhibited EMT by suppressing the activation of transcription factors Snail and Slug, which are correlated with significantly reduced pancreatic cancer cell invasion, suggesting that the Hh signaling pathway is involved in early metastasis. These results indicate that activation of the Hh pathway is a common event. Inhibition of the Hh pathway may be a potential molecular target of new therapeutic strategies for pancreatic cancer.

语种英语
WOS记录号WOS:000314905200037
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66115
专题北京大学第一临床医学院_普通外科
作者单位Peking Univ, Dept Gen Surg, Hosp 1, Beijing 100034, Peoples R China
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GB/T 7714
Hao, Kun,Tian, Xiao-Dong,Qin, Chang-Fu,et al. Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis[J]. ONCOLOGY REPORTS,2013,29(3):1124-1132.
APA Hao, Kun,Tian, Xiao-Dong,Qin, Chang-Fu,Xie, Xue-Hai,&Yang, Yin-Mo.(2013).Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis.ONCOLOGY REPORTS,29(3),1124-1132.
MLA Hao, Kun,et al."Hedgehog signaling pathway regulates human pancreatic cancer cell proliferation and metastasis".ONCOLOGY REPORTS 29.3(2013):1124-1132.
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