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学科主题: 基础医学
题名:
Occurrence of poly-N-acetyllactosamine synthesis in Sf-9 cells upon transfection of individual human beta-1,4-galactosyltransferase I, II, III, IV, V and VI cDNAs
作者: Sato, T; Guo, SC; Furukawa, K
关键词: beta-1,4-galactosyltransferases I-VI ; poly-N-acetyllactosamine ; Sf-9 cells
刊名: BIOCHIMIE
发表日期: 2001-08-01
卷: 83, 期:8, 页:719-725
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: BETA-4-GALACTOSYLTRANSFERASE GENE FAMILY ; ASPARAGINE-LINKED OLIGOSACCHARIDES ; HAMSTER-KIDNEY CELLS ; INSECT CELLS ; UDP-GALACTOSE ; MAMMALIAN BETA-1,4-GALACTOSYLTRANSFERASE ; ACETYLGLUCOSAMINIDASES CII ; EXTENSION ENZYME ; SUGAR CHAINS ; O-GLYCANS
英文摘要:

Lectin blot analysis of membrane glycoprotein samples from Sf-9 cells upon transfection of individual human beta -1,4-galactosyltransferase (beta -1,4-GalT) I, II, III, IV, V et VI cDNAs showed that the endogenous N-linked oligosaccharides are galactosylated (Guo et al., Glycobiology (2001), in press). Further analysis revealed that membrane glycoprotein samples from all the gene-transfected cells are also reactive to Lycopersicon esculentum agglutinin (LEA) et Datura stramonium agglutinin (DSA), both of which bind to oligosaccharides with poly-N-acetyllactosamine chains while no lectin reactive protein bands are detected when blots are pretreated with a mixture of diplococcal beta -1,4-galactosidase et jack bean beta -N-acetylhexosaminidase or N-glycanase. Analysis of endo-beta -galactosidase-digestion products revealed the presence of the Gall --> GlcNAcl --> Gal and/or GlcNAcl --> Gal structures in the gene-transfected cells. When the homogenates of the gene-transfected cells were used as enzyme sources towards oligosaccharides with the GlcNAcP1 --> (3Gal beta1 --> 4GlcNAc)(1-3) structures, human recombinant beta -1,4-GalTs I et II galactosylated these oligosaccharides more effectively than other beta -1,4-GalTs. These results indicate that beta -1,4-GalTs I-VI can synthesize poly-N-acetyllactosamine chains with beta -1,3-N-acetylglucosaminyltransferase. (C) 2001 Societe francaise de biochimie et biologie moleculaire/Editions scientifiques et medicales Elsevier SAS. All rights reserved.

语种: 英语
WOS记录号: WOS:000170846600003
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66121
Appears in Collections:基础医学院_病理学系_期刊论文

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Occurrence of poly-N-acetyllactosamine synthesis in Sf-9 cells upon transfection of individual human beta-1,4-galactosyltransferase I, II, III, IV, V and VI cDNAs.pdf(113KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 1730015, Japan
2.Beijing Med Univ, Dept Pathol, Beijing 100083, Peoples R China

Recommended Citation:
Sato, T,Guo, SC,Furukawa, K. Occurrence of poly-N-acetyllactosamine synthesis in Sf-9 cells upon transfection of individual human beta-1,4-galactosyltransferase I, II, III, IV, V and VI cDNAs[J]. BIOCHIMIE,2001,83(8):719-725.
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