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学科主题: 临床医学
题名:
Polyethylene Glycol-Modified Pigment Epithelial-Derived Factor: New Prospects for Treatment of Retinal Neovascularization
作者: Bai, Yu-Jing1; Huang, Lv-Zhen1; Xu, Xiao-Lei2; Du, Wei1; Zhou, Ai-Yi1; Yu, Wen-Zhen1; Li, Xiao-Xin1
刊名: JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
发表日期: 2012-07-01
DOI: 10.1124/jpet.112.192575
卷: 342, 期:1, 页:131-139
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; MACULAR DEGENERATION ; GENE-TRANSFER ; DELIVERY ; ANGIOGENESIS ; RETINOPATHY ; PHARMACEUTICALS ; PEGYLATION ; PROTEINS ; DRUGS
英文摘要:

Pathological retinal neovascularization and choroidal neovascularization are major causes of vision loss in a variety of clinical conditions, such as retinopathy of prematurity, age-related macular degeneration, and diabetic retinopathy. Pigment epithelial-derived factor (PEDF) has been found to be the most potent natural, endogenous inhibitor of neovascularization, but its application is restricted because of its instability and short half-life. Polyethylene glycol (PEG) has been used as a drug carrier to slow clearance rate for decades. The present study investigated PEGylated-PEDF for the first time and evaluated its long-term effects on preventing angiogenesis in vitro and in vivo. PEG showed lower cytotoxicity to human umbilical vein endothelial cells (HUVECs). In vitro, PEGylated-PEDF inhibited HUVEC proliferation, migration, tube formation, and vascular endothelium growth factor secretion and induced HUVEC apoptosis in a dose-dependent manner, and it showed a statistically significant difference compared with the PEDF treatment group. In vivo, PEGylated-PEDF had a long-lasting effect in both plasma and retinal concentrations. In an oxygen-induced retinopathy model, one intravitreous injection of PEGylated-PEDF after mouse pups were moved into room air resulted in a significant difference in the inhibition of retinal neovascularization, which decreased the nonperfusion area, compared with the PEDF-treated group. Our present study demonstrated for the first time the long-term inhibitory effects of PEGylated-PEDF on the prevention of neovascularization in vitro and in vivo. These data suggest that PEGylated-PEDF could offer an innovative therapeutic strategy for preventing retinal neovascularization.

语种: 英语
所属项目编号: 2011 CB510200
项目资助者: National Basic Research Program of China&prime ; s 973 Program
WOS记录号: WOS:000305395400014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66139
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Minist Educ,Key Lab Vis Loss & Restorat, Beijing 100044, Peoples R China
2.Dong Xiao Jiang Beijing Sci & Technol Co Ltd, Beijing, Peoples R China

Recommended Citation:
Bai, Yu-Jing,Huang, Lv-Zhen,Xu, Xiao-Lei,et al. Polyethylene Glycol-Modified Pigment Epithelial-Derived Factor: New Prospects for Treatment of Retinal Neovascularization[J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,2012,342(1):131-139.
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