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学科主题: 临床医学
题名:
Integrin-linked kinase in gastric cancer cell attachment, invasion and tumor growth
作者: Zhao, Gang1; Guo, Li-Li2; Xu, Jing-Yong1; Yang, Hua1; Huang, Mei-Xiong1; Xiao, Gang1
关键词: Gastric cancer ; Integrin-linked kinase ; Small interfering RNA ; Cell attachment ; Cell proliferation ; Cell invasion ; Cell microfilament dynamics ; Vascular endothelial growth factor ; Nude mice
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2011-08-14
DOI: 10.3748/wjg.v17.i30.3487
卷: 17, 期:30, 页:3487-3496
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: GLYCOGEN-SYNTHASE KINASE ; AP-1 TRANSCRIPTION FACTOR ; PROTEIN-KINASE ; SUPPRESSOR PTEN ; ILK ; ACTIVATION ; INHIBITION ; ADHESION ; EXPRESSION ; B/AKT
英文摘要:

AIM: To investigate the effects of integrin-linked kinase (ILK) on gastric cancer cells both in vitro and in vivo.

METHODS: ILK small interfering RNA (siRNA) was transfected into human gastric cancer BGC-823 cells and ILK expression was monitored by real-time quantitative polymerase chain reaction, Western blotting analysis and immunocytochemistry. Cell attachment, proliferation, invasion, microfilament dynamics and the secretion of vascular endothelial growth factor (VEGF) were also measured. Gastric cancer cells treated with ILK siRNA were subcutaneously transplanted into nude mice and tumor growth was assessed.

RESULTS: Both ILK mRNA and protein levels were significantly down-regulated by ILK siRNA in human gastric cancer cells. This significantly inhibited cell attachment, proliferation and invasion. The knockdown of ILK also disturbed F-actin assembly and reduced VEGF secretion in conditioned medium by 40% (P < 0.05). Four weeks after injection of ILK siRNA-transfected gastric cancer cells into nude mice, tumor volume and weight were significantly reduced compared with that of tumors induced by cells treated with non-silencing siRNA or by untreated cells (P < 0.05).

CONCLUSION: Targeting ILK with siRNA suppresses the growth of gastric cancer cells both in vitro and in vivo. ILK plays an important role in gastric cancer progression. (C) 2011 Baishideng. All rights reserved.

语种: 英语
项目资助者: Ministry of Human Resources and Social Security of China
WOS记录号: WOS:000295003100003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66149
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Beijing Hosp, Dept Gen Surg, Beijing 100730, Peoples R China
2.Peking Univ, Dept Ophthalmol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Zhao, Gang,Guo, Li-Li,Xu, Jing-Yong,et al. Integrin-linked kinase in gastric cancer cell attachment, invasion and tumor growth[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2011,17(30):3487-3496.
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