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A dumbbell molecular beacon for the specific recognition of nucleic acids
Lv, Cong1; Yu, Lili1,2; Wang, Jie1; Tang, XinJing1
关键词Fluorescent probes Structured probes Mismatch detection Oligonucleotides FRET
刊名BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
2010-11-15
DOI10.1016/j.bmcl.2010.09.047
20期:22页:6547-6550
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Medicinal ; Chemistry, Organic
研究领域[WOS]Pharmacology & Pharmacy ; Chemistry
关键词[WOS]OLIGONUCLEOTIDE PROBES ; DNA PROBES ; HYBRIDIZATION ; FLUORESCENCE ; SINGLE
英文摘要

A dumbbell molecular beacon (DMB) was designed and synthesized with the attachment of a fluorophore and a quencher at two ends. This DMB probe can be used to detect single mismatch of a 20mer oligodeoxynucleotide in two different buffers and discrimination factors were as high as 60 at 37 degrees C. Statistics of single substitutions of analytes showed that both substituted positions and substituted nucleotides have important contributions for this probe to efficiently distinguish the true analyte from mismatched ones. Hybridization kinetics of DMB with the target oligonucleotide was also studied. (C) 2010 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000283052900041
项目编号bmu2009137-121 ; 20090101
资助机构Peking University ; State Key Laboratory
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66151
专题北京大学药学院
北京大学医学部管理机构_医学部
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Shaanxi Univ Sci & Technol, Coll Chem & Chem Engn, Xian 710021, Shaanxi, Peoples R China
推荐引用方式
GB/T 7714
Lv, Cong,Yu, Lili,Wang, Jie,et al. A dumbbell molecular beacon for the specific recognition of nucleic acids[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2010,20(22):6547-6550.
APA Lv, Cong,Yu, Lili,Wang, Jie,&Tang, XinJing.(2010).A dumbbell molecular beacon for the specific recognition of nucleic acids.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,20(22),6547-6550.
MLA Lv, Cong,et al."A dumbbell molecular beacon for the specific recognition of nucleic acids".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 20.22(2010):6547-6550.
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