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学科主题: 药学
题名:
A dumbbell molecular beacon for the specific recognition of nucleic acids
作者: Lv, Cong1; Yu, Lili1,2; Wang, Jie1; Tang, XinJing1
关键词: Fluorescent probes ; Structured probes ; Mismatch detection ; Oligonucleotides ; FRET
刊名: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
发表日期: 2010-11-15
DOI: 10.1016/j.bmcl.2010.09.047
卷: 20, 期:22, 页:6547-6550
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Chemistry, Organic
研究领域[WOS]: Pharmacology & Pharmacy ; Chemistry
关键词[WOS]: OLIGONUCLEOTIDE PROBES ; DNA PROBES ; HYBRIDIZATION ; FLUORESCENCE ; SINGLE
英文摘要:

A dumbbell molecular beacon (DMB) was designed and synthesized with the attachment of a fluorophore and a quencher at two ends. This DMB probe can be used to detect single mismatch of a 20mer oligodeoxynucleotide in two different buffers and discrimination factors were as high as 60 at 37 degrees C. Statistics of single substitutions of analytes showed that both substituted positions and substituted nucleotides have important contributions for this probe to efficiently distinguish the true analyte from mismatched ones. Hybridization kinetics of DMB with the target oligonucleotide was also studied. (C) 2010 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: bmu2009137-121 ; 20090101
项目资助者: Peking University ; State Key Laboratory
WOS记录号: WOS:000283052900041
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66151
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Shaanxi Univ Sci & Technol, Coll Chem & Chem Engn, Xian 710021, Shaanxi, Peoples R China

Recommended Citation:
Lv, Cong,Yu, Lili,Wang, Jie,et al. A dumbbell molecular beacon for the specific recognition of nucleic acids[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2010,20(22):6547-6550.
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