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学科主题: 临床医学
题名:
Lentivirus-mediated RNA interference of vascular endothelial growth factor in monkey eyes with iris neovascularization
作者: Yuan, Meng-Ke; Tao, Yong; Yu, Wen-Zhen; Kai, Wang; Jiang, Yan-Rong
刊名: MOLECULAR VISION
发表日期: 2010-08-25
卷: 16, 期:187-89, 页:1743-1753
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]: RETINAL VEIN OCCLUSION ; OCULAR ANGIOGENESIS ; TUMOR LYMPHANGIOGENESIS ; DIABETIC-RETINOPATHY ; IN-VIVO ; VEGF ; EXPRESSION ; GLAUCOMA ; INHIBITION ; MODEL
英文摘要:

Purpose: To explore the in vivo anti-angiogenesis effects resulting from lentivirus-mediated RNAi of vascular endothelial growth factor (VEGF) in monkeys with iris neovascularization (INV).

Methods: Five specific recombinant lentiviral vectors for RNA interference, targeting Macaca mulatta VEGFA, were designed and the one with best knock down efficacy (LV-GFP-VEGFi1) in H1299 cells and RF/6A cells was selected by real-time PCR for in vivo use. A laser-induced retinal vein occlusion model was established in one eye of seven cynomolgus monkeys. In monkeys number1, 3, and 5 (Group 1), the virus (1x10(8) particles) was intravitreally injected into the preretinal space of the animal′s eye immediately after laser coagulation; and in monkeys number 2, 4, and 6 (Group 2), the virus (1x10(8) particles) was injected at 10 days after laser coagulation. In monkey number 7, a blank control injection was performed. In monkeys number 1 and 2, virus without RNAi sequence was used; in monkeys number 3 and 4, virus with nonspecific RNAi sequence was used; and in monkeys 5 and 6, LV-GFP-VEGFi1 was used.

Results: In monkey number 5, at 23 days after laser treatment, no obvious INV was observed, while fluorescein angiography of the iris revealed high fluorescence at the margin of pupil and point posterior synechiae. At 50 days after laser treatment, only a slight ectropion uvea was found. However, in the other eyes, obvious INV or hyphema was observed. The densities of new iridic vessels all significantly varied: between monkey number 5 and number 3 (36.01 +/- 4.49/mm(2) versus 48.68 +/- 9.30/mm(2), p=0.025), between monkey number 3 and monkey number 7 (48.68 +/- 9.30/mm(2) versus 74.38 +/- 9.23/mm(2), p=0.002), and between monkey number 5 and number 7 (36.01 +/- 4.49/mm(2) versus 74.38 +/- 9.23/mm(2), p<0.001).

Conclusions: Lentivirus-mediated RNAi of VEGF may be a new strategy to treat iris neovascularization, while further studies are needed to investigate the long-term effect.

语种: 英语
所属项目编号: 7062065 ; RDB2009-42 ; 30901639 ; 2009B04
项目资助者: Beijing Natural Science Foundation ; People&prime ; s Hospital of Peking university ; National Natural Science Foundation of China ; Beijing Novel Program
WOS记录号: WOS:000281341400003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66161
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China

Recommended Citation:
Yuan, Meng-Ke,Tao, Yong,Yu, Wen-Zhen,et al. Lentivirus-mediated RNA interference of vascular endothelial growth factor in monkey eyes with iris neovascularization[J]. MOLECULAR VISION,2010,16(187-89):1743-1753.
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