IR@PKUHSC  > 北京大学第二临床医学院  > 眼科
学科主题临床医学
Lentivirus-mediated RNA interference of vascular endothelial growth factor in monkey eyes with iris neovascularization
Yuan, Meng-Ke; Tao, Yong; Yu, Wen-Zhen; Kai, Wang; Jiang, Yan-Rong
刊名MOLECULAR VISION
2010-08-25
16期:187-89页:1743-1753
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]RETINAL VEIN OCCLUSION ; OCULAR ANGIOGENESIS ; TUMOR LYMPHANGIOGENESIS ; DIABETIC-RETINOPATHY ; IN-VIVO ; VEGF ; EXPRESSION ; GLAUCOMA ; INHIBITION ; MODEL
英文摘要

Purpose: To explore the in vivo anti-angiogenesis effects resulting from lentivirus-mediated RNAi of vascular endothelial growth factor (VEGF) in monkeys with iris neovascularization (INV).

Methods: Five specific recombinant lentiviral vectors for RNA interference, targeting Macaca mulatta VEGFA, were designed and the one with best knock down efficacy (LV-GFP-VEGFi1) in H1299 cells and RF/6A cells was selected by real-time PCR for in vivo use. A laser-induced retinal vein occlusion model was established in one eye of seven cynomolgus monkeys. In monkeys number1, 3, and 5 (Group 1), the virus (1x10(8) particles) was intravitreally injected into the preretinal space of the animal′s eye immediately after laser coagulation; and in monkeys number 2, 4, and 6 (Group 2), the virus (1x10(8) particles) was injected at 10 days after laser coagulation. In monkey number 7, a blank control injection was performed. In monkeys number 1 and 2, virus without RNAi sequence was used; in monkeys number 3 and 4, virus with nonspecific RNAi sequence was used; and in monkeys 5 and 6, LV-GFP-VEGFi1 was used.

Results: In monkey number 5, at 23 days after laser treatment, no obvious INV was observed, while fluorescein angiography of the iris revealed high fluorescence at the margin of pupil and point posterior synechiae. At 50 days after laser treatment, only a slight ectropion uvea was found. However, in the other eyes, obvious INV or hyphema was observed. The densities of new iridic vessels all significantly varied: between monkey number 5 and number 3 (36.01 +/- 4.49/mm(2) versus 48.68 +/- 9.30/mm(2), p=0.025), between monkey number 3 and monkey number 7 (48.68 +/- 9.30/mm(2) versus 74.38 +/- 9.23/mm(2), p=0.002), and between monkey number 5 and number 7 (36.01 +/- 4.49/mm(2) versus 74.38 +/- 9.23/mm(2), p<0.001).

Conclusions: Lentivirus-mediated RNAi of VEGF may be a new strategy to treat iris neovascularization, while further studies are needed to investigate the long-term effect.

语种英语
WOS记录号WOS:000281341400003
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66161
专题北京大学第二临床医学院_眼科
作者单位Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Yuan, Meng-Ke,Tao, Yong,Yu, Wen-Zhen,et al. Lentivirus-mediated RNA interference of vascular endothelial growth factor in monkey eyes with iris neovascularization[J]. MOLECULAR VISION,2010,16(187-89):1743-1753.
APA Yuan, Meng-Ke,Tao, Yong,Yu, Wen-Zhen,Kai, Wang,&Jiang, Yan-Rong.(2010).Lentivirus-mediated RNA interference of vascular endothelial growth factor in monkey eyes with iris neovascularization.MOLECULAR VISION,16(187-89),1743-1753.
MLA Yuan, Meng-Ke,et al."Lentivirus-mediated RNA interference of vascular endothelial growth factor in monkey eyes with iris neovascularization".MOLECULAR VISION 16.187-89(2010):1743-1753.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yuan, Meng-Ke]的文章
[Tao, Yong]的文章
[Yu, Wen-Zhen]的文章
百度学术
百度学术中相似的文章
[Yuan, Meng-Ke]的文章
[Tao, Yong]的文章
[Yu, Wen-Zhen]的文章
必应学术
必应学术中相似的文章
[Yuan, Meng-Ke]的文章
[Tao, Yong]的文章
[Yu, Wen-Zhen]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。