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学科主题: 临床医学
题名:
Establishment of a flow cytometric assay for determination of human platelet glycoprotein VI based on a mouse polyclonal antibody
作者: Qu, Chen-xue; Wang, Jian-zhong; Wan, Wen-hui; Li, Chuan-bao; Wu, Shu-Ian
关键词: platelet ; glycoprotein VI ; polyclonal antibody BJ010
刊名: JOURNAL OF CLINICAL LABORATORY ANALYSIS
发表日期: 2006
DOI: 10.1002/jcla.20150
卷: 20, 期:6, 页:250-254
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medical Laboratory Technology
研究领域[WOS]: Medical Laboratory Technology
关键词[WOS]: COLLAGEN ; PATIENT ; RECEPTOR ; GPVI ; AGGREGATION ; DEFICIENCY ; RESPONSES ; ADHESION ; DOMAIN
英文摘要:

Glycoprotein VI (GPVI) is the major signaling receptor for collagen on platelets. Recent studies suggest that the surface density of GPVI is related to the activation of platelets by collagen. To measure the level of GPVI on platelets, a mouse polyclonal antibody BJ010 was prepared using an amplified fragment of extracellular domain in GPVI. The specific reactivity of BJ010 was identified by anti-GPVI specific monoclonal antibody 11a12 using immunoprecipitation, sandwich enzyme-linked immunosorbent assay (ELISA) and flow cytometry. The antibody BJ010 recognized both native and denatured human GPVI so that it was used to set up a flow cytometric assay to detect the level of GPVI in normal subjects. The relative level of GPVI on platelets was detected in 101 healthy donors. The median geometric mean fluorescence intensity (GMFI) of platelet GPVI level was 57.7. The variation between minimum and maximum values of platelet GPVI and integrin alpha 2 beta 1 were found to be 3.5- and 4.1-fold, respectively, in the normal subjects. There was a week correlation between the amount of GPVI and integrin alpha 2 beta 1 on platelet surfaces. For the method, the intraassay and interassay coefficient of variation was 6.3% and 8.8%, respectively. The flow cytometric assay described here provides a simple, reliable, reproducible, and readily available means of quantitation of collagen receptor GPVI density on the platelet surface in a larger number of blood samples.

语种: 英语
WOS记录号: WOS:000242490900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66188
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, First Hosp, Dept Clin Lab, Beijing 100034, Peoples R China
2.Peking Univ, First Hosp, Dept Hematol, Beijing 100034, Peoples R China
3.Peking Univ, Oncology Hosp, Dept Clin Lab, Beijing 100871, Peoples R China

Recommended Citation:
Qu, Chen-xue,Wang, Jian-zhong,Wan, Wen-hui,et al. Establishment of a flow cytometric assay for determination of human platelet glycoprotein VI based on a mouse polyclonal antibody[J]. JOURNAL OF CLINICAL LABORATORY ANALYSIS,2006,20(6):250-254.
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