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学科主题: 基础医学
题名:
Comparison of three methods for cryopreservation of human embryonic stem cells
作者: Li, Yang1,2; Tan, Ji-chun3; Li, Ling-song1,2
关键词: Human embryonic stem cell ; cryopreservation methods ; cryopreservation efficiency
刊名: FERTILITY AND STERILITY
发表日期: 2010-02-01
DOI: 10.1016/j.fertnstert.2008.10.052
卷: 93, 期:3, 页:999-1005
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: HUMAN BLASTOCYSTS ; MOUSE EMBRYOS ; VITRIFICATION ; LINES
英文摘要:

Objective: To establish reliable methods for cryopreservation of human embryonic stem cells (hESCs).

Design: Prospective experimental study.

Setting: University laboratory.

Patient(s): One hESC line.

Intervention(S): The attachment rates and recovery rates of cryopreserved hESCs using three different cryopreservation methods were compared.

Main Outcome Measure(s): The hESCs were frozen and thawed by conventional cryopreservation, programmable cryopreservation, and vitrification method. The efficiency of cryopreservation was assessed by attachment rate and recovery rate.

Result(S): The attachment rate and recovery rate after thawing of hESCs frozen by the conventional cryopreservation method were significantly lower than those of hESCs frozen by programmed cryopreservation and vitrification methods. Vitrification resulted in the highest attachment rate and recovery rate compared with the other two methods. Human ESCs after vitrification and programmable cryopreservation still expressed pluripotent markers, maintained normal karyotype, and retained their pluripotency.

Conclusion(s): Our data show that program in able cryopreservation and vitrification methods are appropriate for cryopreservation of hESCs, whereas the conventional slow-rate freezing method is not appropriate for cryopreservation of hESCs. (Fertil Steril (R) 2010;93:999-1005. (C)2010 by American Society for Reproductive Medicine.)

语种: 英语
所属项目编号: 2006CB943603 ; 2006CB503905 ; 2006AA02A114 ; D0750701350704
项目资助者: Chinese National 973 Project ; Chinese National 863 Project ; Natural Science foundation of the Beijing Ministry of Science and Technology
WOS记录号: WOS:000274957300046
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66195
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100083, Peoples R China
3.Shenyang Gynecol & Obstet Hosp, Reprod Ctr, Shenyang, Peoples R China

Recommended Citation:
Li, Yang,Tan, Ji-chun,Li, Ling-song. Comparison of three methods for cryopreservation of human embryonic stem cells[J]. FERTILITY AND STERILITY,2010,93(3):999-1005.
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