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学科主题: 口腔医学
题名:
Dose-dependent enhancement of bone marrow stromal cells adhesion, spreading and osteogenic differentiation on atmospheric plasma-treated poly(l-lactic acid) nanofibers
作者: Liu, Wentao1,2; Cai, Qing3; Zhang, Feng4; Wei, Yan1; Zhang, Xuehui5; Wang, Ying1; Deng, Xuming2; Deng, Xuliang1
关键词: plasma treatment ; poly(l-lactic acid) nanofibers ; Biocompatibility ; surface modification ; osteogenic differentiation
刊名: JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
发表日期: 2013-09-01
DOI: 10.1177/0883911513494623
卷: 28, 期:5, 页:453-467
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Materials Science, Biomaterials ; Polymer Science
研究领域[WOS]: Biotechnology & Applied Microbiology ; Materials Science ; Polymer Science
关键词[WOS]: MESENCHYMAL STEM-CELLS ; DIELECTRIC BARRIER DISCHARGE ; SURFACE MODIFICATION ; CYTOSKELETAL TENSION ; INTEGRIN BINDING ; SCAFFOLDS ; SHAPE ; PERFORMANCE ; SUBSTRATE ; DESIGN
英文摘要:

Although poly(l-lactic acid) nanofibers are known to promote osteogenic differentiation of bone marrow stromal cells, their relative hydrophobicity and surface inertia tend to hinder their biomedical application. We explored a feasible and effective technique to improve the bioactivity and biocompatibility of poly(l-lactic acid) fibers for further application in regenerative medicine. A low-temperature atmospheric plasma was used to treat poly(l-lactic acid) nanofibers for 1, 5, and 10 min, and the surface properties and dose-dependent effects on the behavior of bone marrow stromal cells were studied. Both the amino group content and surface hydrophilicity of the nanofibers increased with treatment time, whereas the spreading and proliferation of bone marrow stromal cells were greatest on nanofibers which had been treated for 5 min, followed by samples treated for 1 and 10 min. The quantitative reverse transcription-polymerase chain reaction analysis of the bone marrow stromal cells on the 5-min-treated nanofibers had the highest expression level of osteogenic marker genes including RUNX2, BMP2, ALP, COL1A1, OPN, and OCN. The nanofibers treated for 5 min also promoted the high levels of alkaline phosphatase activity. These results suggest the exertion of dose-dependent effects by atmospheric plasma treatment on the surface of poly(l-lactic acid) nanofibers, and that this treatment is a feasible and effective technique to improve biomaterial biocompatibility and promotion of osteogenic differentiation of bone marrow stromal cells.

语种: 英语
所属项目编号: 2012CB933900 ; 81171000 ; 2012AA022501
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China
WOS记录号: WOS:000324398600004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66209
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Jilin Univ, Coll Vet Med, Changchun 130023, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China
3.Beijing Univ Chem Technol, Minist Educ, Key Lab Carbon Fiber & Funct Polymers, Beijing 100029, Peoples R China
4.Beijing Armed Police Force, Hosp 2, Oral Clin, Beijing, Peoples R China
5.Tsinghua Univ, Dept Mat Sci & Engn, Beijing, Peoples R China

Recommended Citation:
Liu, Wentao,Cai, Qing,Zhang, Feng,et al. Dose-dependent enhancement of bone marrow stromal cells adhesion, spreading and osteogenic differentiation on atmospheric plasma-treated poly(l-lactic acid) nanofibers[J]. JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS,2013,28(5):453-467.
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