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学科主题: 基础医学
题名:
cGMP-dependent protein kinase in regulation of basal tone and in nitroglycerin- and nitric-oxide-induced relaxation in porcine coronary artery
作者: Qin, Xue; Zheng, Xiaoxu; Qi, Hui; Dou, Dou; Raj, J. Usha; Gao, Yuansheng
关键词: cGMP ; basal tension ; relaxation ; Rp-8-Br-PET-cGMPS ; myristoylated PKI
刊名: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
发表日期: 2007-09-01
DOI: 10.1007/s00424-007-0249-8
卷: 454, 期:6, 页:913-923
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Physiology
研究领域[WOS]: Physiology
关键词[WOS]: CYCLIC-NUCLEOTIDE ANALOGS ; TRACHEAL SMOOTH-MUSCLE ; GUANYLYL CYCLASE ; PULMONARY VEINS ; DEFICIENT MICE ; L-ARGININE ; CAMP ; GMP ; ATHEROSCLEROSIS ; INHIBITION
英文摘要:

Cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG) may act as a critical enzyme for nitric-oxide-induced vasodilation. In this study, the role of PKG in regulation of basal tension and in relaxation induced by nitrovasodilators in coronary arteries was determined. Under basal conditions, Rp-8-Br-PET-cGMPS, a specific PKG inhibitor, evoked a significant contraction of isolated porcine coronary arteries, which was prevented by nitro-L-arginine or the removal of the endothelium. Relaxation to nitroglycerin and (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate (DETA NONOate) in vessels preconstricted with U46619 was largely abolished by 1H-[1,2,4]oxadiazolo[4,3]quinoxalin-1-one (ODQ) and inhibited by 48 to 79% by Rp-8-Br-PET-cGMPS. Relaxation of the vessels to 8-Br-cGMP was inhibited by 56% by Rp-8-Br-PET-cGMPS. The basal activity of PKG but not that of cyclic adenosine monophosphate-dependent protein kinase (PKA) was inhibited by nitro-L-arginine, ODQ, or Rp-8-Br-PET-cGMPS. The activity of PKG but not that of PKA was increased by nitroglycerin and DETA NONOate in intact vessels and increased by cGMP in the tissue homogenates. These effects were abolished by Rp-8-Br-PET-cGMPS but not by myristoylated PKI, a specific inhibitor of PKA. These results suggest that in porcine coronary arteries, PKG is involved in the regulation of basal tension and plays a primary role in relaxation induced by nitrovasodilators, whereas PKA may play a minor role.

语种: 英语
WOS记录号: WOS:000249122100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66240
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
2.Peking Univ, Minist Educ Res, Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China
3.Univ Calif Los Angeles, Harbor Med Ctr, Div Neonatol, Torrance, CA 90509 USA

Recommended Citation:
Qin, Xue,Zheng, Xiaoxu,Qi, Hui,et al. cGMP-dependent protein kinase in regulation of basal tone and in nitroglycerin- and nitric-oxide-induced relaxation in porcine coronary artery[J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY,2007,454(6):913-923.
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