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学科主题: 临床医学
题名:
Examination of AKAP12 promoter methylation in skin cancer using methylation-sensitive high-resolution melting analysis
作者: Wu, W.4; Zhang, J.2; Yang, H.3; Shao, Y.2; Yu, B.1,2
刊名: CLINICAL AND EXPERIMENTAL DERMATOLOGY
发表日期: 2011-06-01
DOI: 10.1111/j.1365-2230.2010.03968.x
卷: 36, 期:4, 页:381-385
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dermatology
研究领域[WOS]: Dermatology
关键词[WOS]: DNA METHYLATION ; HIGH-THROUGHPUT ; CELL-LINES ; MALIGNANCIES ; STAGE ; PCR
英文摘要:

P>Background.

A kinase anchor protein 12 (AKAP12/gravin) belongs to a family of scaffold proteins and organizes protein kinase (PK)A and PKC. DNA hypermethylation in the AKAP12 promoter region has been reported in a variety of human cancers with the exception of skin cancer. Methylation-specific high-resolution melting (MS-HRM) analysis is a novel tool for analysis of promoter methylation.

Aim.

To use MS-HRM analysis to detect the methylation levels of the AKAP12 gene in skin samples.

Methods.

In total, 195 samples, including basal cell carcinoma, squamous cell carcinoma and actinic keratosis were examined. MS-HRM analysis was used to detect methylation levels of the AKAP12 gene in these samples.

Results.

MS-HRM analysis successfully detected the methylation of AKAP12 in skin samples. The frequencies of AKAP12 methylation in all three types of skin abnormalities were significantly higher than in normal tissues.

Conclusions.

Application of MS-HRM analysis proved to be a fast and high-throughput method to investigate the epigenetic status of AKAP12. Methylation of AKAP12 can be detected in different skin abnormalities.

语种: 英语
所属项目编号: 200901013
项目资助者: Shenzhen Research Grants
WOS记录号: WOS:000290584100011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66246
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Shenzhen Key Lab Translat Med Dermatol, Shenzhen PKU HKUST Med Ctr, Shenzhen, Guangdong, Peoples R China
2.Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen, Guangdong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Clin Lab, Shenzhen, Guangdong, Peoples R China
4.Fudan Univ, Dept Dermatol, Huashan Hosp, Shanghai 200433, Peoples R China

Recommended Citation:
Wu, W.,Zhang, J.,Yang, H.,et al. Examination of AKAP12 promoter methylation in skin cancer using methylation-sensitive high-resolution melting analysis[J]. CLINICAL AND EXPERIMENTAL DERMATOLOGY,2011,36(4):381-385.
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